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All Journal Biosaintifika: Journal of Biology & Biology Education Biosaintifika: Journal of Biology & Biology Education
Ning Setiati
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Indonesia
Agriculture, Biological Sciences & Forestry

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The Density and Viability of Metarhizium anisopliae Conidia on Several Growth Media Dyah Rini Indriyanti; Siti Harnina Bintari; Ning Setiati; Jamil Maulana Zahriyan Alfiyan
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.31408

Abstract

Metarhizium anisopliae is a parasitic fungus on insects, and thus called entomopathogenic fungus. This fungus is used as a biological control agent for insect pests. Fungal propagation can be done using a variety of media. The purpose of this study was to analyze the growth of M. anisopliae on four types of media, with conidial density and viability as the growth parameters. This research was conducted at the Laboratory of Microbiology, Universitas Negeri Semarang. This study was an experimental research used a Completely Randomized Design (CRD) with one factor and four treatments: Control (PDA/Potato Dextrose Agar medium), Treatment I (ELSA/Extract Larvae Sucrose Agar medium), Treatment II (CWSA/Coconut Water Sucrose Agar medium), Treatment III (CWELSA/Coconut Water and Extract Larvae Sucrose Agar medium). The and results showed that there was an effect of growth media on the density and viability of M. anisopliae conidia. CWELSA media had the highest conidial density (2.91 x 108 cfu/mL) and viability (97.17%). CWSA media had  2.82 x 108 cfu/mL and 95.33%. PDA media had 2.25 x 108 cfu/mL and 92.83%. ELSA media had 1.64 x 108 cfu/mL and 90.83%. The high conidial density and viability of M. anisopliae is CWELSA  medium. This study is as an alternative growth medium to improve the quality of M. anisopliae propagation.
Insecticidal Activity of Gamal (Gliricidia sepium) Leaf Extracts Against the Darkling Beetle Alphitobius diaperinus  Priyantini Widiyaningrum; Wibowo Faria Fadillah Nur; Dyah Rini Indriyanti; Ning Setiati
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.18770

Abstract

Alphitobius diaperinus is a well-known insect pest in broiler farms. Larvae and adults of A. diaperinus act as disease vectors for poultry, and their presence has the potential to increase the risk of carcass contamination in slaughterhouses. Chemical insecticide control is still considered ineffective and environmentally unfriendly. Plant-based insecticide formulations offer a more environmentally friendly approach to pest control compared to chemical insecticides. The ethanol extract of Gamal leaves was tested on the darkling beetle A. diaperinus to determine its insecticidal activity. Five pairs of A. diaperinus were treated at five levels of extract concentration and then maintained in an insect container with wheat bran (pollard) food for 5 weeks. The insecticidal efficacy was measured based on the difference in mortality in the toxicity test and the reproductive ability of adult A. diaperinus by counting the number of larvae produced during the rearing period. The results showed that differences in extract concentrations significantly affected toxicity and the number of larvae produced. The more concentrated the extract, the higher the insect mortality, with the estimated LC50 value of the extract being 20.80% concentration within 72 hours. Reproductive ability showed that the higher the concentration of the extract, causing a decrease in the number of larvae produced, with the optimum concentration found in the 80% treatment. Thus, Gamal leaf extract has potential as a botanical insecticide for biological control of A. diaperinus pest.
Co-Authors Dyah Rini Indriyanti Jamil Maulana Zahriyan Alfiyan Priyantini Widiyaningrum Siti Harnina Bintari Wibowo Faria Fadillah Nur