Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Biosaintifika: Journal of Biology & Biology Education Annales Bogorienses
Tiwit Widowati
Research Center for Biotechnology, Indonesian Institute of Sciences, Indonesia
Agriculture, Biological Sciences & Forestry

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Isolation, Screening and Identification of Plant Growth-Promoting Endophytic Bacteria from Theobroma cacao Rumella Simarmata; Tiwit Widowati; Tirta Kumala Dewi; Sylvia J. R. Lekatompessy; Sarjiya Antonius
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.21280

Abstract

Cacao (Theobrama cacao) is one of the main plantation commodities in Indonesia which has an important role for the national economy. The low productivity of cacao plants in Indonesia is due to the condition of old plants, pests and diseases attacks which affect the quality of the fruit and decrease the plant’s productivity. The objective of this study was to isolate and identify of endophytic bacteria from stem, flower, leaf and fruit of T. cacao by using 16S rRNA gene as genetic marker. Twenty seven endophytic bacterial isolates were collected from local plantation in Yogyakarta area.  From this study, 8 endophytic bacterial strains exhibited the higher PGP traits. The isolates produced Indole Acetic Acid level by 0.3 to 5.21 ppm/hour. All of isolates had nitrogen fixation activity but have not phosphate solubilization activity. Among them, isolates CSDT 4 and CGKBH 4 showed promising potential as PGP bacteria. Based on the 16S rRNA gene sequences, those bacterial strains were identified as Brevibacillus brevis (CSDT 4) and Pantoea sp. (CGKBH 4). We propose that the B. brevis and Pantoea sp. which is reported for the first time for their PGP potential in cacao, exerts its beneficial effects on cacao crop through combined of activities. The potential PGP bacteria from the Cacao plant was used to make a specific bio-fertilizer formula for the Cacao plant, because of the different needs and condition that every plant requires.
POTENTIAL OF GROWTH HORMONES IAA OF ENDOPHITIC MICROBES FROM NEEM PLANTS FOR CAPITAL OF INDUSTRIAL OPPORTUNITY Sylvia J R Lekatompessy; Nuriyanah; Liseu Nurjanah; Eleazar Handoyo; Rumella Simarmata; Tiwit Widowati
Annales Bogorienses Vol. 25 No. 1 (2021)
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2021.v25.n1.41-54

Abstract

Indonesia has a tropical climate that is rich in diversity of microorganisms with useful potential and has important values that can be developed in the fields of industry, health and agriculture. Neem is a plant commonly used as an insecticide for pest control. Biotechnology continues to develop in exploiting the unique and interesting potential of endophytic microbes to obtain useful bioactive compounds extracted from plants, without having to take large amounts of plant biomass. This study aims to explore other potentials of endophytic microbes from neem plants, namely as a producer of IAA growth hormone to support plant growth. The results of the test of growth hormone-producing microbes, from 19 isolates of endophytic bacteria tested, obtained 3 isolates of IAA hormone-producing bacteria. 36 isolates of endophytic fungi were tested, 18 isolates of endophytic fungi were positive for IAA hormone production. One of the isolates of endophytic fungi that produces the highest IAA hormone, has been identified molecularly and the result is Colletotrichum gloeosporioides. The potential value derived from the endophytic microbes of neem plants can be developed into biotechnology products that have high commercial value by utilizing potential genes to support plant growth.
Co-Authors Eleazar Handoyo Liseu Nurjanah Nuriyanah Rumella Simarmata Rumella Simarmata Sarjiya Antonius Sylvia J R Lekatompessy Sylvia J. R. Lekatompessy Tirta Kumala Dewi