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All Journal Biosaintifika: Journal of Biology & Biology Education
Mochammad Muchlish Adie
Indonesian Legume and Tuber Crops Research Institute, Indonesia
Agriculture, Biological Sciences & Forestry

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The Effect of Seed Position in Pod on The Seed Viability of Cowpea (Vigna unguiculata) Mochammad Muchlish Adie; Ayda Krisnawati; Ratri Tri Hapsari
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.17730

Abstract

In Indonesia, cowpea (Vigna unguiculata L. Walp) is widely cultivated in the dry climate area. Famers used the seeds from previous plants or traditional markets. The aims of the study were to determine the effect of seed position in the pods on seed viability and to study the seed and seedling characteristics regarding the genetic variation and seed position. The cowpea used in this study was from 18 Indonesian local varieties. Twenty-five matured pods were randomly detached from each variety. Each pod was divided into three parts, i.e. the lower third (base), the middle third, and the upper third (tip). The seeds of each part were germinated using sand media to evaluate their viability. Each local variety has different response to the percentage of seed viability in each seed position in the pod. The highest abnormal germination occurred in the seed from lower part of the pod, followed by seed in the middle of the pod and the tip of the pod. The variation of seed viability in pods was more determined by genetic differences in local varieties. The average seed viability from three parts of the pods was highest for seeds originated from local varieties of Lumajang and Sampang. Therefore, it is recommended that the seeds of both local varieties can be mixed to be used for optimum cowpea cultivation. 
Assessment of Soybean Resistance to Leaf-feeding Insect, Spodoptera litura Ayda Krisnawati; Nuryati Nuryati; Mochammad Muchlish Adie
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.24794

Abstract

One of soybean problem in Indonesia is leaf damage caused by the Spodoptera litura. The aim of the study was to identify the resistance of soybean genotypes againts army worm based on the preference index and morphophysiological characters. 150 soybean genotypes were grown under field condition from February to May 2018 in Malang (East Java, Indonesia). The preference test was conducted in the laboratory using G100H as resistant reference plant to armyworm. The result showed that the preference index was ranged from 0.09-1.82. A total of 105 genotypes showed a low preference index (resistant), 39 genotypes showed a high preference index (susceptible), and a genotype has similar preference index with the reference plant (has equal resistance with G100H). Based on the preference index and morphophysiological characters, 150 soybean genotypes can be grouped into eight clusters. A genotype from cluster VIII (Anjasmoro/Rajabasa-41) has characteristics of low leaf water content, dense leaf trichomes, and the preference index of 0.14. Those characteristics could be the attributes of the soybean genotypes that is resistant to S. litura. The Anjasmoro/Rajabasa-41 was recommended to be cultivated in Indonesian, or may be used as a gene source in the breeding program for S. litura resistance
Co-Authors Ayda Krisnawati Nuryati Nuryati Ratri Tri Hapsari