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All Journal Biosaintifika: Journal of Biology & Biology Education
Al Azhar
Faculty of Veterinary Medicine, Universitas Syiah Kuala
Agriculture, Biological Sciences & Forestry

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Neurogenic Differentiation of Bone Marrow Mesenchymal-Like Stem Cell Induced by Delonix regia Flowers Extract Kartini Eriani; Irma Suryani; Al Azhar; Risa Nursanti; Ichsan Ichsan; Arief Boediono
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.15051

Abstract

Stem cell technology has great potential in the effort to cure degenerative diseases. This study was done to determine optimum dose of flamboyant (Delonix regia) flower extract to induce proliferation and differentiation of mice (Mus musculus) bone marrow mesenchymal-like stem cell. Bone marrow cells were collected from mice by aspiration. Cells suspension (1 x 106) were poured into petri dishes containing 2 ml of modified Dulbecco's Modified Eagle's Media (mDMEM) and incubated overnight at 37 °C in a 5% CO2 incubator and microscopically observed. In quadriplicate, MSC were cultured in mDMEM containing D. regia flower extract of 0.0 (control), 0.4, 0.6, 0.8, and 1.0 mg/ml and incubated at 37 °C for 9 days. Population doubling time (PDT) and differentiated cell type were microscopically observed using HE staining on day 1 and 10. Data obtained were analyzed by ANOVA and Tukey test. The results showed that the addition of D regia flowers extracts 0.8 and 1.0 mg/ml significantly reduced PDT compared to that of 0.4, 0.6 and control. The extract, at 0.4 and 0.6 mg/ml, were able to induce MSC differentiation into fibroblast-like and nerve-like cells. In conclusion, D. regia flower extracts of 0.6, 0.8 and 1.0 mg/ml were able to stimulate MSC proliferation, but optimum dose for neurogenic differentiation was 0.6 mg/ml. This is the first to show potential of D. regia flower extract as neurogenic differentiatian inducer on mice MSC. These findings can be used as preliminary information for using the extract as cellular differentian inducer in basic and applicative reseach using stem cells.
Alkaline Phosphatase Expression From Mice Mesenchymal Stem Cells Induced By Flamboyant Flower (Delonix regia) Extract Kartini Eriani; Deby Anggraini; Yudha Bintoro; Ichsan Ichsan; Al Azhar; Silmi Mariya
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25433

Abstract

Flamboyant flower (Delonix regia) extract can increase proliferation and differentiation rates of mesenchymal stem cells (MSCs) into specific cells such as bone, nerve, and fibroblast cells. The extract possess metabolic compounds that may act as antibiotics, anti-inflammatory, antimicrobial, diuretic, anthelmentic, astringent, leucorrhoea, and potentially increase the body's metabolism normally. This study aimed to investigate expression level of alkaline phosphatase (ALP) by mice MSCs treated with flamboyant flower extract in vitro. Here, mice bone marrow cell cultures were treated with flamboyant flower extracts of 0.6 mg/ml (P1), 0.7 mg/ml (P2), 0.8 mg/ml (P3), and 0.9 mg/ml (P4). Untreated cell culture was used as negative control (P0). Expression of ALP gene was measured by RT-qPCR method. The results showed that mice mesenchymal stem cell could differentiate into bone, nerve, and fibroblast cells. The addition of flamboyant flower extract ranged from 0.6-0.9 mg/ml significantly (p0.05) influenced the expression of ALP by differentiating MSCs. The highest expression was found at the stem cells treated with flamboyant flower extract of 0.8 mg/ml, 0.13 times compared with control. In conclusion, flamboyant flower extracts treatment might increase the expression of ALP in differentiating MSCs.  This information can be used as a basis for finding an appropriate biomarkers for tracking the differentiation and profileration of tissue originated MSCs induced by extracts of medicinal plants.
Co-Authors Daniel Happy Putra Deby Anggraini Ichsan Ichsan Ichsan Ichsan Irma Suryani Kartini Eriani Kartini Eriani Risa Nursanti Silmi Mariya Yudha Bintoro