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All Journal Acta Aquatica: Aquatic Sciences Journal
N Ira Sari
Departemen Teknologi hasil Perikanan Fakultas Perikanan dan Kelautan Universitas Riau
Materials Science & Nanotechnology Other

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Characteristic of gelatin bone tilapia (Oreochromis nilaticus) extracted with acid and base Andarini Diharmi; Helmi Nurhanif; N Ira Sari
Acta Aquatica: Aquatic Sciences Journal Vol. 9 No. 2 (2022): Acta Aquatica: Aquatic Sciences Journal
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v9i2.6159

Abstract

This study was aimed to determine chemical and physical characteristics of extracted tilapia fish bone gelatin using acid (HCl) and alkaline (NaOH) solutions. The research method was used comparative experimental with Paired t-test consisting of 2 treatments, namely 4% HCl and 4% NaOH with 3 replications. Analysis parameters was consisted of yield, gel strength, viscosity, pH, moisture content, and ash. Characteristics of gelatin extracted using acid solution was resulted in yield, gel strength and viscosity was 4.40, 182.65 g/bloom. and 9.90 cP. Characteristic of gelatin extracted with acid has moisture, ash and pH levels of 5.85%, 2.35%, and 4.25. Gelatin extracted with bases was produced physical characteristics of yield, gel strength, and viscosity of 4.67%, 150.41 g/bloom and 6.03 cP and chemical characteristics (moisture, ash, and pH) were 5.77%, 3.37%, and 10.24. The extraction process using acids and bases resulted in tilapia fish bone gelatin with significantly different characteristics on gel strength, viscosity, pH, and ash except yield and moisture content. Gelatin was produced from tilapia fish bones using acids has gel strength, pH, ash, and moisture content in accordance with SNI standards except for viscosity, while gelatin uses bases had gel strength, viscosity and moisture content, except for pH and ash.Keywords: characteristic physico-chemical; gel strength; pH; viscosity; yield
Identification of bioactive compounds in extract fraction red seaweed (Eucheuma cottonii) Indra Agusman; Andarini Diharmi; N Ira Sari
Acta Aquatica: Aquatic Sciences Journal Vol. 9 No. 2 (2022): Acta Aquatica: Aquatic Sciences Journal
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v9i2.6164

Abstract

Seaweed is a biological resource that is rich in nutrients and bioactive compounds. This study aims to determine qualitatively and quantitatively the bioactive compounds (flavonoids, alkaloids, steroids/terpenoids, saponins and phenols) from extracts and fractions of Eucheuma cottonii. The method used is an experiment by conducting a series of experiments by extracting and fractionating E. cottonii and the data were analyzed descriptively. The treatments used were organic solvents with different polarity levels (methanol, n-hexane, ethyl acetate, and butanol) for extraction and fractionation. Parameter analysis consisted of qualitative phytochemical tests (flavonoids, alkaloids, steroids/terpenoids, saponins and phenols) and quantitatively determined the total content of alkaloids, flavonoids, and saponins. The results of the phytochemical test of methanol extract qualitatively produced alkaloids, flavonoids, steroids/terpenoids, and saponins. The n-hexane fraction contains steroids/terpenoids and saponins. The ethyl acetate fraction contains alkaloids, flavonoids and steroids/terpenoids and the butanol fraction contains alkaloids. The results of quantitative analysis of the n-hexane extract fraction showed that the content of saponins was 0.23%, alkaloids and flavonoids were 0.16% and 0.09% in the ethyl acetate fraction, while the alkaloid content was 0.09% in the butanol fraction.Keywords: fractionation; organic solvent; phytocemical; quantitative
Co-Authors Andarini Diharmi Helmi Nurhanif Indra Agusman