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All Journal Journal of Indonesian Medical Laboratory and Science (JoIMedLabS)
Agus Rudi Hartono
Progam Studi D-III Teknologi Laboratorium Medis, Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Kaltim, Provinsi Kalimantan Timur, Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology

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PENGARUH PENYIMPANAN REAGEN KERJA TERHADAP AKTIVITAS ENZIM ALANINE AMINOTRANSFERASE Rafika Saeba Delfiana; Tiara Dini Harlita; Agus Rudi Hartono
Journal of Indonesian Medical Laboratory and Science Vol 4 No 2: Oktober 2023
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v4i2.187

Abstract

The activity of the Alanine Aminotransferase (ALT) enzyme in working reagents stored at 2 – 8°C will affect results of laboratory tests. Influencing factors are temperature and storage time. The aim of research was to determine effect of storage treatment working reagents with aliquots and without aliquots for 0, 7, 14 and 21 days at temperature 4°C on ALT enzyme activity. Research used Quasi Experimental, with Randomized Group Design. Research variables, the storage treatment of the ALT working reagent and the level of ALT enzyme activity. Sample used was 2,400 mL normal ALT control serum. Examination method is enzymatic using spectrophotometer. Data analysis used univariate analysis to calculate the average enzyme levels examined 3 times and bivariate analysis to test effect of storage treatment with Independent Sample T-test. Based on the average aliquot treatment content, highest yield on day 0, namely (59.0 U/L) and lowest yield on day 21, namely (45.7 U/L). Treatment without aliquots highest yield on day 0, namely (54.6 U/L) and lowest yield on day 21, namely (51.8 U/L). Results of treatment effect test have a significant value, aliquots can be used for up to 3 weeks and without aliquots can be used for up to 4 weeks.
Co-Authors Rafika Saeba Delfiana Tiara Dini Harlita