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All Journal Jurnal Kimia Mulawarman Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat IJOEP : International Journal of Ecophysiology Jurnal Akademika Kimia JOURNAL OF CHEMICAL NATURAL RESOURCES (JCNaR) Jurnal Akademika Kimia
Tarigan, Juliati
Department Of Chemistry, Faculty Of Mathematic And Natural Sciences, Universitas Sumatera Utara, Medan 20155, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Social Sciences

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Journal : JOURNAL OF CHEMICAL NATURAL RESOURCES (JCNaR)

 1 
Analysis of Melamine on Powdered Milk-Based Diazoation Reactions with Eugenol Reagent By Spectrophotometry Juliati Br Tarigan
Journal of Chemical Natural Resources Vol. 2 No. 1 (2020): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (467.937 KB) | DOI: 10.32734/jcnar.v2i1.9313

Abstract

The analysis of melamine on the milk-based diazotization reaction with eugenol was carried out. The presence of primary aromatic amine on melamine was reacted to sodium nitrite, while chloride acid formed diazonium salt, and then diazonium salt reacts with eugenol to form a colored azo compound. The absorbance was measured spectrophotometrically at a maximum wavelength of 470 nm. Analysis of melamine on the sample milk was determined by using a standard addition method. In this research, the optimization of concentration and incubation time was observed at the maximum formation of azo dyes. The optimization results were obtained at a concentration of eugenol of 9.10-3M, NaNO2 of 9.10-4 M, HCl of 0.25 M, and an incubation time of 5 minutes. The content of melamine in the milk sample was 2.513.10-5 M.
Co-Authors Aditia Warman Cut Fatimah Zuhra Firman Sebayang Hana Melyana Pertiwi Manurung Jamaran Kaban Junedi Ginting REZA ZULMI Sahfitri W. Nasution