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All Journal COJ (Coastal and Ocean Journal)
Endah Sri Rahayu
Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, IPB University, 16680, Indonesia
Agriculture, Biological Sciences & Forestry Environmental Science Social Sciences

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DNA BARCODING UNTUK VALIDASI SPESIES PAUS SPERMA (Physeter macrocephalus LINNAEUS, 1758) DARI PERAIRAN LAUT SAWU, NUSA TENGGARA TIMUR, INDONESIA Agus Alim Hakim; Endah Sri Rahayu; Ali Mashar; Nurlisa Alias Butet; Yusli Wardiatno; Mohammad Mukhlis Kamal
Coastal and Ocean Journal (COJ) Vol 7 No 1 (2023): COJ (Coastal and Ocean Journal)
Publisher : Pusat Kajian Sumberdaya Pesisir dan Lautan IPB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/coj.v7i1.49702

Abstract

The sperm whale (Physeter macrocephalus) is a protected marine biota in Indonesia. Molecular identification of sperm whales from the Sawu Sea (East Nusa Tenggara, Indonesia) was carried out using the 16S rRNA gene. This study aims to validate the species of the sperm whale (Physeter macrocephalus) in Indonesia using the partial cytochrome oxidase subunit I (COI) gene. The specimens used were tissue specimens from laboratory collections taken from Sawu Sea waters in 2014. The three samples were validated as Physeter microcephalus with 100% similarity. The base composition is dominated by the base thymine (31.7–31.8%) with a GC content of 42.7–42.8%. The genetic distance for the same species was 0.0015, while the highest genetic distance for Orcaella brevirostris was 0.2128. Molecular identification using the 16S rRNA gene and COI has validated the species of a sperm whale (Physeter macrocephalus) in Indonesia so that this genetic data can be used as a basis for sperm whale resource management and conservation.
Co-Authors Agus Alim Hakim Ali Mashar Mohammad Mukhlis Kamal Nurlisa Alias Butet Yusli Wardiatno