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All Journal Jurnal Kelautan Tropis
Sri Sedjati
Department of Marine Sciences, Faculty of Fisheries and Marine Sciences, Universitas Diponegoro

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Study on the anti-vibrio activity of marine fungi Aspergillus sydowii and Rhizopus sp. using OSMAC Approach Galank Fad'qul Janarkho; Agus Trianto; Sri Sedjati; Rindiani Puja Listari
Jurnal Kelautan Tropis Vol 26, No 3 (2023): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v26i3.20451

Abstract

Shrimp is one of the major aquaculture products in Indonesia. However, shrimp culture faces the peril of Vibriosis, a disease caused by the bacteria genus of Vibrio.  Marine sponge-associate microbes are recognized for their potential as sources of antibacterial agent. The fungus Aspergillus sydowii isolate that used in this research originally isolated from marine sponge collected in Lampung Bay. The fungus was grown in various media e. g. Malt extract agar (MEA), MEA-Tempeh broth (MEA-T), and tofu dregs (TD) under various pH (5.5, 7.5, and 9.5).  The fungus also be culture as mono-culture and co-culture with Rhizopus sp. The anti-vibrio assay was conducted using disk diffusion method. Based on anti-vibrio assay, the fungus A. sydowii and Rhizopus sp. didn’t show any correlation with the anti-vibrio compound produced. The fungus Rhizopus sp. cultivated in tofu dregs media (TD) at pH 5.5 exhibited the highest potential for inhibiting against V. alginolyticus (5.85±0.24 mm), V. harveyi (5.20±0.20 mm), and V. vulnificus (4.33±0.15 mm), while the co-culture (A. sydowii and Rhizopus sp.) in TD media and pH 7.5 against V. parahaemolyticus (5.55±0.86 mm). The fungus cultured in pH 7.5 can promotes the potential inhibition zone than a pH 9.5. 
Natural Pigment Screening: Comparative Analysis of Chlorophyll and Carotenoid Content and Antioxidant Potential in Various Seaweed Species Sri Sedjati; Wilis Ari Setyati; Ali Ridlo; Intan Swastika Sari; Tiara Rahmawati
Jurnal Kelautan Tropis Vol 29, No 1 (2026): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v29i1.28463

Abstract

The present study aimed to evaluate the levels of chlorophyll and carotenoids, as well as the antioxidant potential of various seaweed species collected from the waters of Kartini Beach, Jepara, Central Java, Indonesia. Nine seaweed samples were extracted using methanol, and the pigment content was determined using spectrophotometric analysis. The antioxidant activity of the extracts was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. The findings indicated that the green seaweed Caulerpa serrulata exhibited the highest concentrations of chlorophyll a (489.42 µg/g) and carotenoids (248.83 µg/g). Conversely, the brown seaweed Sargassum echinocarpum demonstrated superior antioxidant properties, with DPPH radical scavenging activities of 92.59%, followed by C. serrulata extracts at 76.43%.  The antioxidant activity of these extracts exceeded that of ascorbic acid at a concentration of 20 µg/mL (71.21%). A moderate positive correlation was observed between pigment content and antioxidant activity. The variations in pigment composition among different seaweed species significantly influenced their antioxidant potential, with carotenoid-rich brown seaweeds exhibiting higher antioxidant activity compared to green seaweeds. The study's findings reveal the potential of C. serrulata and S. echinocarpum extracts as natural pigments with antioxidant properties. These extracts offer promising applications in the food, nutraceutical, and cosmetic industries as safe and sustainable alternatives to synthetic antioxidants and colorants.
Co-Authors Agus Trianto Ali Ridlo Galank Fad'qul Janarkho Intan Swastika Sari Rindiani Puja Listari Tiara Rahmawati Wilis Ari Setyati