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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Asian Journal of Health and Applied Sciences
Fadhilah, Wasiyah Khusna
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health

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Comparison of hemoglobin level measurement results using sodium lauryl sulphate with Oshiro and Mansoor procedure Fadhilah, Wasiyah Khusna; Al Badri, Muhammad Hilal; Wahid, Aziz Ansori; Fadhila, Feldha
Asian Journal of Health and Applied Sciences Vol. 1 No. 1 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.919 KB) | DOI: 10.53402/ajhas.v1i1.4

Abstract

The method of measuring Hb levels recommended by ICSH uses the HiCN method. However, it has a high risk due to the toxicity of the reagent. Therefore, the determination of Hb has been developed using the SLS method. According to Oshiro and Mansoor, who both stated that there was no significant difference between SLS and HiCN, this method has two distinct procedures. This study aims to determine the comparison between the measurement results of hemoglobin levels using sodium lauryl sulfate with the Oshiro procedure and Mansoor. The research design used is analytical research. The data obtained from the results of measuring Hb levels with the Oshiro and Manshoor procedures was given to students of the Diploma IV Study Program of Medical Laboratory Technology, Rajawali Health Institute batch 2018, which collected 49 people. The sampling technique used was saturated sampling to reduce the error rate in the study. The results of the normality test of the data in this study showed n of the two procedures was 49 mean±SD for the Oshiro procedure 13.09±0.56 and p = 0.200. Meanwhile, the Mansoor procedure had a meanSD of 13.09±0.57 and a p-value of 0.059. Because the probability of both being p > 0.05, the data is declared normally distributed. The results of the average difference test from the data of this study show the mean±SD of the pairwise difference between the Oshiro and Mansoor procedures is 0.002±0.059 and the p value = 0.811. If P > 0.05, then it is stated that there is no significant difference between the two groups. The conclusion of this study is that the result of measuring Hb levels using the SLS procedure is reliable. Oshiro and Mansoor conform to HiCN and there is no significant difference in the mean results.
Mung beans (Phaseolus radiates L.): Utilization as components of the growth medium Bacillus subtilis Fadhilah, Wasiyah Khusna; Saputra, Heru Agung; Baby, Nimisha
Asian Journal of Health and Applied Sciences Vol. 1 No. 2 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53402/ajhas.v1i2.35

Abstract

Bacillus subtilis is a bacterial contaminant widely discovered in laboratories and used in educational needs. Synthetic media are relatively expensive, which makes it difficult for microbiology laboratories to meet large-scale bacterial growth media demands. This has encouraged researchers to find alternative media with cheaper and easily available prices, namely Mung Beans, which are known to contain protein nutrients that are good for bacterial growth, which is as much as 24%. The purpose of this study was to determine the potential of mung beans’ alternative media as a medium for bacterial growth. This research is a descriptive study and the sample used is 1 tube of a pure isolate of Bacillus subtilis. The variable of this research is mung beans as an alternative medium for the growth of Bacillus subtilis. The results were obtained by observed macroscopically and microscopically, followed by a carbohydrate fermentation test and an IMViC test. Then showed that the colonies growing on the alternative medium of mung beans were in accordance with the characteristics of the colonies growing on Nutrient Agar medium (control), which indicated that these characteristics were Bacillus subtilis. Based on the research that has been done, it can be concluded that there is a qualitative growth of Bacillus subtilis in mung beans alternative media, which means that mung beans alternative media has the potential as a growth medium for Bacillus subtilis.
Analysis of Marketing Mix Relationships on Patient Satisfaction and Loyalty in the Laboratory Unit of X Hospital in 2022 Fadhilah, Wasiyah Khusna; Katmini, Katmini
Asian Journal of Health and Applied Sciences Vol. 2 No. 1 (2023): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53402/ajhas.v2i1.180

Abstract

A healthcare facility, one of which is a hospital, must practice management more quickly as the environment changes due to global competition. In order to sustain client loyalty and satisfaction, X Hospital must maintain and enhance its services. Companies must devise the best marketing strategy to deal with the challenge of the Service Marketing Mix's influence on consumer purchase decisions. Especially for service marketing (7p) in the form of products, prices, promotions, places, people, processes and physical evidence. The hospital has important medical support, one of which is a laboratory. The laboratory unit has not conducted a service satisfaction survey. Conducting a service survey is crucial as a critical component of medical diagnosis. This is also based on the 2019 X Hospital's first-semester periodic report, 49.95% of patients received laboratory services. This amount fell short of the predetermined goal. Further investigation is required to determine how patient loyalty and satisfaction in the laboratory unit of X Hospital might be impacted by the analysis of marketing mix relationships. This research aims to analyse the relationship between the marketing mix and patient satisfaction and loyalty in the laboratory unit of X Hospital in 2022. The method employed is analytic quantitative with a cross-sectional approach. The result of the direct influence value is 0.077 and the indirect effect is 0.556, meaning that the indirect effect value is greater than the direct influence value. The research's conclusion there is a relationship between the marketing mix and customer satisfaction and loyalty at X Hospital's laboratory unit in 2022.
Formulation and Nutrient Component Analysis of Various Cost-Effective Bacterial Culture Media Based on Horticultural Waste Handarini; Sopiyani, Putri; Farizal, Deni; MK, Alby Salman Al Farizi; Fadhilah, Wasiyah Khusna
Jurnal Penelitian Pendidikan IPA Vol 11 No 12 (2025): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i12.13545

Abstract

Horticultural waste, which is often overlooked, has great potential as an alternative raw material for cost-effective and environmentally friendly culture media. This study aims to develop a cost-effective alternative bacterial culture media formulation by utilizing horticultural waste. In addition, this study also aims to analyze the nutritional components of the formulated media to ensure its effectiveness in supporting bacterial growth. The results showed that E. coli was able to grow on all types of test media. This indicates slower growth compared to the control media. The average number of E. coli colonies on BW Agar and CW Agar at concentrations of 10%, 15%, and 20% after incubation for 48 hours was >300 CFU/mL. Meanwhile, the average number of E. coli colonies on PW Agar at concentrations of 10%, 15%, and 20% was 126 × 10⁷ CFU/mL, 171 × 10⁷ CFU/mL, and 225 × 10⁷ CFU/mL, respectively. The number of S. aureus colonies was only obtained on BW Agar with concentrations of 10%, 15%, and 20%, namely 77 × 10⁷ CFU/mL on BW Agar 10%, 200 × 10⁷ CFU/mL on BW Agar 15%, and 300 × 10⁷ CFU/mL on BW Agar 20%. Based on these results, the optimal formulations representing each type of test media selected for nutrient content analysis were BW Agar 20%, CW Agar 20%, and PW Agar 20%.
Co-Authors Al Badri, Muhammad Hilal Baby, Nimisha Fadhila, Feldha Farizal, Deni Handarini Heru Agung Saputra Katmini, Katmini MK, Alby Salman Al Farizi Sopiyani, Putri Wahid, Aziz Ansori