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All Journal Heca Journal of Applied Sciences Grimsa Journal of Science Engineering and Technology
Trina Ekawati Tallei
Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado 95115, Indonesia
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Engineering Health Professions Medicine & Pharmacology Physics

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GC-MS Analysis Reveals Unique Chemical Composition of Blumea balsamifera (L.) DC in Ie-Jue Geothermal Area Nur Balqis Maulydia; Khairan Khairan; Trina Ekawati Tallei; Ethiene Castellucci Estevam; Mohsina Patwekar; Fazlin Mohd Fauzi; Rinaldi Idroes
Grimsa Journal of Science Engineering and Technology Vol. 1 No. 1 (2023): October 2023
Publisher : Graha Primera Saintifika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61975/gjset.v1i1.6

Abstract

Blumea balsamifera (L.) DC. or Sembung is a flowering plant belonging to the genus Blumea of the family Asteraceae. Many pharmacological activities of this plant show potential in human therapy. In this study, an investigation was conducted on the ethanolic extract of B. balsamifera collected from a geothermal area known as Ie-Jue, in Aceh Province, Indonesia. The results showed that the ethanolic extract of B. balsamifera contained secondary metabolites of flavonoids and tannins. Chemical constituents of ethanolic extracts B. balsamifera further analysis using gas chromatography-mass spectrometry (GC-MS) show that active compounds from this plant was Proximadiol (C15H28O2) with relative area 41.76%. This research underscores the compelling potential of the Ie-Jue geothermal area as a promising reservoir of flora owing to the plant's adaptability to geothermal extremities.
Beyond Morphology: COI-Based Molecular Identification of Sea Urchins from Kumu Beach, North Sulawesi Nur Aisyah; Trina Ekawati Tallei; Beivy Jonathan Kolondam
Heca Journal of Applied Sciences Vol. 4 No. 2 (2026): September 2026
Publisher : Heca Sentra Analitika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60084/hjas.v4i2.451

Abstract

Sea urchins (Echinoidea) play important ecological and economic roles in coastal ecosystems, but morphological identification is often unreliable due to overlapping diagnostic traits among closely related taxa. This study used DNA barcoding of the mitochondrial COI gene to identify a sea urchin specimen from Kumu Beach, North Sulawesi, and determine its phylogenetic relationship with GenBank reference sequences. DNA was extracted from gonad tissue, amplified via PCR using the BK-COI-AntF&AntR primers, sequenced, and analyzed through BLAST, MUSCLE alignment, and Maximum Likelihood phylogenetic reconstruction (Kimura 2 Parameter model, 1000 bootstrap replications). PCR yielded a clear ~709 bp fragment, producing a 658 bp COI consensus sequence (A+T content: 56.84%). BLAST analysis showed 100% query cover and identity with multiple Tripneustes gratilla reference sequences, and phylogenetic analysis placed the specimen within the Tripneustes clade, closely nested among T. gratilla sequences. These results confirm the specimen as T. gratilla and support COI based DNA barcoding as a reliable method for sea urchin species identification.
Co-Authors Beivy Jonathan Kolondam Ethiene Castellucci Estevam Fazlin Mohd Fauzi Khairan Khairan Mohsina Patwekar Nur Aisyah Nur Balqis Maulydia Rinaldi Idroes