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Determination of vitamin D3 encapsulated in liprotide β-lactoglobulin crosslink oleic acid by HPLC and SEM Sylvia Rahmi Putri; Gemala Anjani; Faizah Fulyani; Diana Nur Afifah; Ahmad Syauqy; Fitriyono Ayustaningwarno
AcTion: Aceh Nutrition Journal Vol 8, No 4 (2023): December
Publisher : Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/action.v8i4.1290

Abstract

Vitamin D is an unstable compound to light, heat, and certain chemicals, so it is easily degraded under various conditions. Therefore, instability is achieved through the encapsulation method using complex protein carrier compounds with fatty acids known as liprotide. This study aims to determine vitamin D3. Method, the determination of vitamin D3 was analyzed, including the determination of vitamin D3 standard eluents, calibration curve, and determining the retention time of vitamin D3 encapsulated in liprotide using HPLC (High-Performance Liquid Chromatography). Morphology of vitamin D3 encapsulated in liprotide using SEM (Scanning Electron Microscopy). The results showed that the standard vitamin D3 HPLC analysis using acetonitrile: methanol and acetonitrile: aquabidest obtained two peaks. Butanol: n-hexane eluent obtained one peak with a retention time of 2,170 minutes. The results of the standard calibration curve for vitamin D3 at a linearity value of R2 = 0,9997 and f(x) = 14,928x-117,930. The same retention time was obtained for vitamin D3 encapsulated in liprotide. Conclusion, an enlargement of the cubic structure, which occurred due to the encapsulation of vitamin D3 by the liprotide-forming micelles.
Perilaku makan dengan kejadian stunting pada balita: Studi cross-sectional di Kecamatan Nabire Papua Eka Cahyaningsih Wulandari; Ani Margawati; Ahmad Syauqy; Bagoes Widjanarko; Mohammad Zen Rahfiludin
Jurnal SAGO Gizi dan Kesehatan Vol 5, No 3A (2024): Agustus
Publisher : Poltekkes Kemenkes Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/gikes.v5i3A.1798

Abstract

Background: Nabire is an area that has more stunting nutrition problems with a percentage of 17.1%. Feeding style by parents need to be considered because it will affect behavior and nutritional intake that support the growth and development of toddlers will provide opportunities for stunting.Objectives: The study aimed to measure the relationship of eating behavior based on parental feeding style with the incidence of stunting in toddlers aged 12 to 59 months in Nabire District, Papua.Methods: This study was conducted observational with cross-sectional design. Subjects were collected using purposive sampling method. Research has been conducted at Karang Tumaritis, Bumiwonorejo, Nabire Kota, and Siriwini Health Centers, in October-December 2023. The sample was 106 toddlers, ages 12-59 months who  were taken by non-probability sampling. Data collection on eating behavior based on Parental Feeding Style Questionnaire (PSFQ)  and toddler height or body length data measured through anthropometric measurement. Data were analyzed using Chi-square method CI 95%.Results: The results of the study found 46,2% of toddlers were stunted and most respondents (56,6%) had inappropriate eating behavior. The relationship test showed a significant relationship between the incidence of stunting and eating behavior based on parental feeding style (p=0,008 and OR 3,200;95%CI=1,422 – 7,201).Conclution: Eating behavior based on parental feeding style has a significant relationship with the incidence of stunting in toddlers.