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The effect of indigenous and exogeneous endophythic bacteria on shoot production, soil and tea leaf nitrogen content under drought season Eko Pranoto
Jurnal Penelitian Teh dan Kina Vol 17 No 1 (2014)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v17i1.27

Abstract

Drought season with rainfall <100 mm/month for more than two months cause tea plant growth disturbed, reduction in leaf pro­duction, and death of plant. Nitrogen was the macro essential nutrient needed for vegetative growth and was important for building the cell, include protein, DNA, and RNA. Nitrogen was related on proline content to support the drought tolerance in plant. The nitrogen re­quirement on tea plantation can be fulfilled by anorganic, organic, and biofertilizer. This study was aimed at determining effect of indigenous and exogeneous endophythic bacteria effec­tiveness on productivity and nitrogen content on soil and tea plant. This research was carried out at the Block A7 Gambung, Research Intitute for Tea and Cinchona. The study was conduc­ted during drought season (June to September 2013). The experimental design was randomize blocked design with factorial arrangement and three levels of each factor: i.e. 1. anorganic nitrogen dose (N): a. 100% N dose; b. 75% N dose; c. 50% N dose, 2. endophytic bacteria (E); a. control (without); b. indigenous (DtG7-5); c. exogenous (Acinetobacter sp). The result showed that the indigenous and exogenous endophytic bacteria could be maintained the leaf production and significantly different on drought season, i.e. on 3rd, 4th, and 6th plucking. The highest leaf production increasing depend on before applied the treatment was the combined of endophitic bacteria and 100% anorganic fertilizer. All of the treatment has not significantly different on N-total of soil and N content of plant. The applied of indigenous and also exogenous endophytic bacteria was pro­duced the average of nitrogen content on soil around 10-13% higher than the control treat­ment (only anorganic fertilizer applied) and the nitrogen content on plant was lower 1-2% than control treatment (only anorganic fertilizer applied).
In-vitro experiment of nitrogen fixing capacity of indigenous and exogeneous Azotobacter sp at Andisol tea planting area’s Eko Pranoto; Mieke Rochimi Setiawati
Jurnal Penelitian Teh dan Kina Vol 17 No 1 (2014)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v17i1.43

Abstract

An in-vitro experiment to evaluate total nitrogen content of soil of tea plantation area included with indigenous and exogenous Azoto­bacter sp have been conducted in the Biology and Soil Biotechnology Laboratory, Padja­djaran University. A completely randomized design with 11 treatments and three repli­cations was used. The treatments were: A (ne­gative control); B (positive control); C (A. Kedelai II); D (A. vinelandii); E (A. N.D.9.3); F (A. Padi); G (VI-1); H (II-1); I (V-2); J (I-1); and K (III-2). Size of microbe inoculated popu­lation for each treatment was 1,2 x 1011 CFU/ml. One percent dose was used for ste­rilized soil originated from the tea plantation area. After five days of incubation period re­vealed that II-1 Azotobacter sp produced very significantly different in total nitrogen pro­duction than the other Azotobacter sp, i.e. 0,8532%. Orthogonal comparisons indicated that indigenous Azotobacter sp had the capacity of nitrogen fixation 7,67% higher than that the capacity of nitrogen fixation of exogenous Azotobacter sp for growth on Andisol soil of tea plantation area with pH of 4,5-5,6. Although, Azotobacter sp is very rarely found in soil with low pH (high acidity), this phenomena indi­cating that an active biofertilizer of indigenous Azotobacter sp is present in tea plantation area.