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Pience Maabuat
Universitas Sam Ratulangi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Estimasi Serapan Karbon Pada Vegetasi Mangrove di Pesisir Pantai Kecamatan Bunaken, Kota Manado, Sulawesi Utara Friska S. Panjaitan; Roni Koneri; Pience Maabuat
JURNAL BIOS LOGOS Vol. 13 No. 3 (2023): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v13i3.53417

Abstract

Abstrak Peningkatan emisi gas rumah kaca menyebabkan pemicu terjadinya pemanasan global yang berdampak terhadap terjadinya perubahan iklim. Berkaitan dengan hal ini, hutan mangrove memiliki fungsi ekologis yang penting bagi kawasan pesisir sebagai penyerap dan penyimpan karbon dalam upaya mitigasi pemanasan global. Penelitian ini bertujuan untuk mengestimasi serapan Karbon pada vegetasi mangrove di Pesisir Pantai Kecamatan Bunaken. Penelitian ini dilakukan pada dua lokasi yang termasuk dalam Kecamatan Bunaken. Metode yang digunakan yaitu non destructive dengan menggunakan persamaan allometrik, dan penenetuan stasiun dilakukan secara purposive sampling dan pembuatan transek dan plot ukuran 10 m x 10 m dan jarak antar plot 10 meter. Hasil penelitian pada dua lokasi didapatkan sebanyak 4 famili, 8 spesies dan 282 individu mangrove. Nilai kandungan biomassa mangrove di Kelurahan Meras sebesar 1892,39 ton/ha, dan nilai serapan karbon sebesar 3246,21 ton/ha. Nilai kandungan biomassa mangrove di Desa Bahowo sebesar 3039,69 ton/ha, dan serapan karbon sebesar 5243,18 ton/ha. Serapan kandungan karbon pada vegetasi mangrove pada lokasi penelitian dipengaruhi oleh kerapatan vegetasi dan diameter batang pohon mangrove.
Identification of Mangrove using DNA Barcoding and Vegetation Analysis at the Sarawet Mangrove Ecotourism Site, North Minahasa Regency Sendy B Rondonuwu; Pience Maabuat; Vany Kamu; Dwi Rahayu Pujiastuti
JURNAL BIOS LOGOS Vol. 16 No. 1 (2026): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v16i1.67260

Abstract

This study aims to identify the diversity of mangrove species in the Mangrove Ecotourism area of Sarawet Village, Likupang, North Minahasa Regency, using a molecular taxonomy approach based on DNA barcoding and an ecological approach through vegetation analysis. Vegetation data collection was conducted descriptively and qualitatively using transect and plot methods. Molecular analysis included DNA extraction from mangrove leaf samples, amplification of the matK and rbcL genes using Polymerase Chain Reaction (PCR), followed by sequencing and nucleotide base comparison using the Basic Local Alignment Search Tool (BLAST). The morphological vegetation analysis found six mangrove species at the research site, namely Rhizophora mucronata, Rhizophora apiculata, Bruguiera sp., Sonneratia alba, Aegiceras corniculatum, and Ceriops tagal. Meanwhile, the molecular identification results of DNA barcoding showed that the matK gene sequence in the SRA sample had a 99.98% similarity with Sonneratia alba, and the rbcL gene sequence in the SRC sample had a 100% similarity with Aegiceras corniculatum. The combination of molecular identification and vegetation analysis provides accurate data on mangrove species composition, which is highly essential as a foundation for monitoring, sustainable management, and conservation of the mangrove ecotourism area as a buffer zone.
Co-Authors Dwi Rahayu Pujiastuti Friska S. Panjaitan Roni Koneri Sendy B Rondonuwu Vany Kamu