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All Journal Advances in Tropical Biodiversity and Environmental Sciences
Helen Joan Lawalata
Manado State University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Isolation of Salmonella sp. from Eggs Commonly Sold in Tondano Market, Minahasa, North Sulawesi Britney Meissy Pandoh; Herry Maurits Sumampouw; Helen Joan Lawalata; Danny Christian Posumah; Anita Constanci Christine Tengker; Yermia Semuel Mokosuli
Advances in Tropical Biodiversity and Environmental Sciences Vol. 9 No. 1 (2025): February 2025: Pages 1-66
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2025.v09.i01.p01

Abstract

Various types of eggs are one of the foodstuffs that are in great demand by the community, especially the people of North Sulawesi, because they are cheap and easy to obtain. Furthermore, eggs have the nutritional content of animal protein, which is good for humans. Numerous studies on eggs have revealed that microorganisms, including Salmonella sp., can contaminate them. This study aims to detect Salmonella sp. bacteria in various types of eggs in the Tondano Market, Minahasa, North Sulawesi. This research uses a descriptive method (qualitative method) to describe an event objectively. We collected up to three samples, the first of which is located outside the market. Three samples of purebred chicken eggs, duck eggs, and quail eggs were taken. Then, the second point inside the market was taken: 3 samples of purebred chicken eggs, duck eggs, and quail eggs from the same trader. To prepare the samples, the eggshells were separated from the insides, then 2 grams of the eggshells were crushed and mixed with 5 ml of Buffered Peptone Water (BPW) 0.1%. This mixture was then added to 10 ml of Rappaport Vassiliadis (RV) media, followed by scraping onto Salmonella Shigella Agar (SSA) media, and then inoculating onto Xylose Lysine Deoxycholate agar (XLD) media. Four eggshell samples tested positive for Salmonella sp. bacteria: duck eggs (TB 1), quail eggs (TP 1), broiler eggs (TA 2), and quail eggs (TP 2), while two samples were negative: broiler eggs (TA 1) and duck eggs (TB 2). In contrast, two samples were negative for Salmonella sp., namely broiler eggs (TA 1) and duck eggs (TB 2).
The Use of Beauveria bassiana as Biological Control against the Pepper Aphid Insect Pest Myzus persicae Sulz Semuel Harpandi; Jantje Ngangi; Helen Joan Lawalata; Sukmarayu Pieter Gedoan; Utari Satiman; Yermia Semuel Mokosuli
Advances in Tropical Biodiversity and Environmental Sciences Vol. 9 No. 1 (2025): February 2025: Pages 1-66
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2025.v09.i01.p02

Abstract

This research is motivated by chilies, which are one of the plant products that become raw materials for daily needs and are therefore classified as a secondary food source. Insect pests are a factor affecting chilli productivity, use of fungi as biological controllers, to determine the use of the fungus Beauveria bassiana as a biological control against the insect pest of chilli aphids Myzus persicae Sulz, the research method used in this study is an experimental method using the complete randomized design (CRD) method with five treatments and three replications, where each plot has five samples, the treatment doses were Control, P1 (6 g/500 ml), P2 (7 g/500 ml), P3 (8 g/500 ml), P4 (9 g/500 ml) and Lethal Dose 50, the results of this study showed that the application of B. bassiana had a significant effect on the mortality of M. persicae Sulz with the mortality obtained was 0.75% of the total number of samples 120 outside the control treatment, so the higher the dose, the higher the mortality, the LD50 result was 6.025 g/ml, showing that the toxicity of B. bassiana falls into the category of mild toxins. Therefore, we can conclude that the fungus B. bassiana serves as an effective biological control agent against the insect pest M. persicae Sulz.
Co-Authors Anita Constanci Christine Tengker Britney Meissy Pandoh Danny Christian Posumah Herry Maurits Sumampouw Jantje Ngangi Mokosuli Yermia Samuel Semuel Harpandi Sukmarayu Pieter Gedoan Utari Satiman