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Priyono .
Pusat Penelitian Kopi dan Kakao Indonesia
Agriculture, Biological Sciences & Forestry

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Analysis of Cutting Growth Characteristics in Robusta Coffee(Coffea canephora Pierre.) Ucu Sumirat; Fitria Yuliasmara; Priyono .
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 29 No 3 (2013)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v29i3.9

Abstract

Development of Robusta coffee clones needs special characteristics for rootstock. This research was aimed to study the characteristics of cutting growth of Robusta coffee as influenced by genetic factor. The research was conducted at Indonesian Coffee and Cocoa Research Institute using 269 progenies originated from reciprocal crossing populations among three parentals, namely BP 409, BP 961, and Q 121. BP 308 an easy cutting-propagated genotype was used as control. The research was arranged in three replications of randomized completely block design with 10 cuttings per replication. Each cutting was single planted in plastic polybag of 15 cm x 25 cm without any growth-regulator treatment. Cluster analysis procedure showed root growth characteristics which could be divided into three groups namely easy (85.3%, 3.82 and 6.68 cm), moderate (57.6%, 1.73 and 4.01 cm) and difficult (25.1%, 0.58 dan 1.44 cm) based on proportion of rooted cuttings, number of primary root and length of root, respectively. On the other side, growth of sprout showed good homogenous characteristic, mainly indicated by proportion of sprouted cuttings which generally achieved up to 98% in average. Study on shoot-root ratio of cuttings resulted in two groups of progenies, namely the first which tended to be dominant on sprout growth and the second which tended to balance their root and sprout growth. Based on proportion of sprouted cuttings which almost achieved up to 100% and various proportion of rooted cutting, it could be indicated that growth of cuttings was started and dominated by the growth of sprout. Key words: Coffee canephora, progeny, genetic variation, cutting, rooting, sprouting
Confirmation of Transgenic Robusta Coffee (Coffea canephora) Transformed by Chitinase-encoding Gene and Its Propagation Through Somatic Embryogenesis Priyono .; Asmini Budiani; Surip Mawardi
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 21 No 2 (2005)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v21i2.16

Abstract

Genetic engineering of Robusta coffee resistant to fungal diseases might be done by introducing a chitinase-encoding gene into genome of this plant. This research was aimed to confirm transgenic plant of BP 308 clone Robusta coffee transformed by chi gene and to evaluate its ability for the somatic embryogenesis. Confirmation of transgenic was carried out by analysis the presence of NPTII gene as a selectable marker for Canamysin resistant using PCR technique. The somatic embryo initiation and reproduction were evaluated in 11 plant accessions. Three kinds of sucrose concentration, 20%, 30% and 40% were applied in initiation stage of somatic embryo germination. The suitability of 4 medium, namely M1 (without addition by liquid medium), M2 (addition by liquid medium contained 0.25 mg/l kinetin), M3 (addition by liquid medium contained 0.25 mg/l IAA) and M4 (addition by liquid medium contained 0.25 mg/l GA3 ) was evaluated for somatic embryo maturation. The result showed that 8 out of 10 plant accessions tested were transgenic and they could be propagated through somatic embryogenesis. The ability of transgenic plant for somatic embryo initiation, reproduction and regeneration were similar with that of nontransgenic one. Germination of somatic embryo could be improved by using 40% sucrose. Maturation of somatic embryo could be improved by addition of fresh liquid medium on the ancient gelled medium that used for somatic embryos reproduction. The best result was obtained on addition of fresh medium contained 0.25 mg/l GA 3 in which 65% of the somatic embryos developed to pre-germinate somatic embryo. Key words: Coffea canephora, transgenic plant, somatic embryogenesis.
Co-Authors Asmini Budiani Fitria Yuliasmara Surip Mawardi Ucu Sumirat