Andisols are characterized by dominance of amorphous minerals which form strong and stable bonding with organic matter, therefore Andisols always contain high organic matter. For that reason, organic fertilizer is generally not applied on Andisols, because it is assumed that it will not give any positive effect on growth or yield. The experiment was aimed to evaluate response of mature Kartika 1 Arabica coffee variety (seven years old) cultivated on Andisols applied with organic matter derived from cow dung manure. The experiment was carried out at Andungsari Experimental Station located in Bondowoso District, East Java. Elevation of the site was 1,150 m asl., with rainfall type of C (Schmidt & Fergusson). The experiment was arranged according to completely randomized block design with four replications to evaluate effect of ninecombination treatments of application rates at application depths of 50, 100, and 150 cm. The range of organic fertilizers rates were 0 - 13.5 kg/tree/year. The experiment revealed that cow dung manure applications on Arabica coffee cultivated on Andisols significantly increased yield at the average of 33% compared to the untreated crop. No significant effect of the treatment onvariables of leaf water deficit and soil moisture content during dry season and root density. At range of application depths of 50 - 150 cm, the deeper the organic matter applications, the higher the yield will be.Key words: Andisols, Arabica coffee, organic matter, cow dung manure