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All Journal Jurnal Florea Flora: Jurnal Kajian Ilmu Pertanian dan Perkebunan
Raden Achmad Djazuli
Jurusan Agribisnis, Fakultas Pertanian, Universitas Muhammadiyah Gresik
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Comparative Expression Analysis of Geraniol Biosynthesis genes in Differents Accessions of Cymbopogon nardus Rofiatun Solekha; Sabilla Khoirotunnisa; Siti Lathifatul Mahmudah; Puput Virnanda Yuniar; R. Achmad Djazuli
Florea : Jurnal Biologi dan Pembelajarannya Vol. 13 No. 1 (2026)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/florea.v13i1.24357

Abstract

Citronella (Cymbopogon nardus) is rich in absorbing flavonoids, and its precious geraniol is superior, none can be compared. Despite having a number of well-known cultivars of citronella in Indonesia with unique bioactive potential, the expression profiles of genes and geraniol profile content across these plants remain unknown. Here we report on the relative expression of crucial biosynthetic and regulatory genes involved in geraniol production, analyzed in relation to β-carotene levels and geraniol content measured from five different Indonesian citronella cultivars. Gene expression was quantified using real-time quantitative PCR (RT-qPCR), targeting nine genes: CnDXS, CnIDI, CnGPPS, CnGES, CnLIS, CnUGT, CnMYB, CnGEDH, and CnGDP. Geraniol content was determined according to High Performance Liquid Chromatography (HPLC). Citrona 1 Agribun and Citrona 2 Agribun showed higher expression of the upstream biosynthetic genes (CnGES, CnGPPS) and the regulatory gene CnMYB for which showed also good linearity with highest geraniol 24.71 mg/L and 26.35 mg/L, respectively. These results demonstrate a positive expression levels and geraniol content, highlighting the transcriptional complexity in citronella geraniol biosynthesis. The conclussion, suggest possible molecular candidates for enhancing contents in biofortification scenarios (e.g., selective breeding or genome editing) for essential oil quality.
Efisiensi Usahatani Padi (Oryza Sativa) Di Dusun Menganti Desa Karangsemanding Kecamatan Balongpanggang Gresik Irvan Maulana; Garist Sekar Tanjung; R. Achmad Djazuli
Flora : Jurnal Kajian Ilmu Pertanian dan Perkebunan Vol. 1 No. 2 (2024): Juni : Flora : Jurnal Kajian Ilmu Pertanian dan Perkebunan
Publisher : Asosiasi Riset Ilmu Tanaman Dan Hewani Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62951/flora.v1i2.56

Abstract

Indonesia is known as an agricultural country, making the agricultural sector very important with the commodity most widely cultivated by the public being rice. Karangsemanding Village is a village that has extensive agricultural land but has a small productivity value compared to other villages. This research aims to determine the factors that influence the production of lowland rice farming and determine the efficiency of lowland rice farming in Menganti Hamlet. The research was conducted in Menganti Hamlet in June-September 2023. Data collection was carried out through interviews and questionnaires as well as secondary data obtained. The sampling technique was saturated sampling which involved all members of the population sampled as many as 86 farmers. Data were analyzed descriptively and in multiple linear regression analysis with the help of SPSS software. The research results show that production factors in the form of land area, seeds, fertilizer, pesticides and labor simultaneously influence rice production, while partially the variables of land area, pesticides and labor have a significant influence on production. The results of the technical efficiency analysis show a value of 0.8894 which is said to be efficient in the use of production factors because it is close to 1. Judging from the price efficiency (allocative) of lowland rice farming, it shows a value of 0.0416, this shows that lowland rice farming in Menganti Hamlet is not yet efficient economically. price. Meanwhile, the economic efficiency of lowland rice farming shows a value of 0.0370, which means lowland rice farming is economically inefficient..
Co-Authors Garist Sekar Tanjung Irvan Maulana Puput Virnanda Yuniar Rofiatun Solekha Sabilla Khoirotunnisa Siti Lathifatul Mahmudah