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Biochemistry, Genetics & Molecular Biology

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Hidrolisis Xilan Bagas Menggunakan Xilanase Bacillus subtilis XJ28 dan Karakterisasi Enzimnya A, Gading Wilda; ., Yopi; Meryandini, Anja
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2865.185 KB) | DOI: 10.14203/jbi.v11i1.2150

Abstract

Xylanase extracellular enzyme is produced by various microbes. Hydrolysis of bagasse xylan can produced xylooligosaccharide. The main components of hemicellulose was xylan at bagasse. IdentificationBacillus subtilisXJ28 using primer 16S RNA. The qualitative test used Congo-Red stainning, whereas quantitative test used Dinitrosalicyclic Acid (DNS) methode. The hydrolyzing product was analysed used TLC (Thin Layer Chromatography). This research aims were to characterized xylanase from isolate XJ28 and analyzing the hidrolyzing product from xylan bagasse. Pretreatment process included delignification using sodium hypochlorite 1% and xylans extraction using alkaline (NaOH 15%) as the solvent. The result of the xylans extracted from bagasse was 9,9% xylan and after purification was obtained 3,4% soluble xylan. Xylanase activity has the highest activity at 96 h of incubation time with activity 11,3 U/mL. Xylanase Bacillus subtilis XJ28has the optimum condition at pH 7 and 50 ºC and stable up to 72 hr of  incubation time at room temperature and 4 ºC. The hydrolysis product using xylanase crude enzyme was reducing sugar with molecular weight around  of sucrose and oligosaccharide. Keywords: xylanase, xylan bagasse, hydrolysis, TLC. 
Optimasi Produksi Enzim Amilase dari Bakteri Laut Jakarta (Arthrobacter arilaitensis)   Purnawan, A.; Capriyanti, Y.; Kurniatin, PA.; Rahmani, N.; ., Yopi
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2195

Abstract

ABSTRACTAmylase is one of the most important industrial enzymes which can be used in a number of industrial processes including food industry, textile, paper industry, renewable energy, and pharmaceutical. This study reports the environmental conditions and nutrient media for amylase production from marine bacterium Arthrobacter arilaitensis. Various parameters such as substrate concentration, pH medium, temperature of fermentation, co-substrate, and nitrogen source was determined to obtain the optimum conditions. Maximum amylase enzyme production was obtained at starch concentration 1%, pH 7, temperature fermentation 30°C, co-substrate maltose with activity 2.7 U/mL. While the addition of several nitrogen sources was given decreased amylase activity, such as addition casein was decreased the activity into 2.3 U/mL. Keywords: Arthrobacter arilaitensis, amylase activity, optimization, co-substrate, pH, temperature, substrate, nitrogen source 
Co-Authors A, Gading Wilda Anja Meryandini Capriyanti, Y. Popi Asri Kurniatin Purnawan, A. Rahmani, N.