Ismed Setya Budi
Prodi Proteksi Tanaman Fakultas Pertanian Universitas Lambung Mangkurat

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Formulasi Bakteri Endofit Untuk Menekan Kejadian Penyakit Fusarium Pada Padi Beras Merah (Oryza nivara. L) Ahmad Khairil Anam; Mariana Mariana; Ismed Setya Budi
JURNAL PROTEKSI TANAMAN TROPIKA Vol 7 No 2 (2024): Vol 7(2): Juni 2024
Publisher : www.ulm.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jptt.v7i2.2606

Abstract

This research aims to study the effect of peat soil endophytic bacterial formulations, lowland swamp soil endophytic bacterial formulations, tidal swamp soil endophytic bacterial formulations, kelakai endophytic bacterial formulations on red rice seeds in suppressing the incidence of fusarium disease. This study used a completely randomized design (CRD) consisting of 5 treatments and 4 replications, namely control inoculation without endophytic bacteria, endophytic bacterial formulation with peat soil, endophytic bacterial formulation with lowland swamp soil, endophytic bacterial formulation with tidal swamp soil, bacterial formulation endophytes with kelakai and added one control inoculation treatment without endophytic bacteria and without pathogens to observe the parameters of plant height and number of tillers. Endophytic bacterial formulation treatment was able to reduce the incidence of fusarium disease. The endophytic bacterial formulation with lowland swamp soil had the lowest disease incidence of 67.5% with an effectiveness of 30.7%, the tidal swamp soil formulation 70% with an effectiveness of 28.2%, the peat soil formulation 77.5% with an effectiveness of 20.5%. % and the kelakai formulation was 82.5% with an effectiveness of 15.3%, while the control had the highest disease incidence of 97.5%. Apart from that, the endophytic bacteria formulation could extend the incubation period. Endophytic bacterial formulations can also increase plant height and number of tillers. Apart from that, soaking seeds with endophytic bacterial formulations does not affect seed germination and length of brown rice sprouts.
Uji Streptomyces sp. Isolat Lahan Rawa Untuk Menekan Pertumbuhan Colletotrichum sp. Asal Cabai Rawit Varietas Hiyung Secara In Vitro Alfi Sahriyanor; Mariana Mariana; Ismed Setya Budi
JURNAL PROTEKSI TANAMAN TROPIKA Vol 7 No 2 (2024): Vol 7(2): Juni 2024
Publisher : www.ulm.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jptt.v7i2.2612

Abstract

Hiyung chili is a local chili variety from South Kalimantan which is often attacked by anthracnose disease caused by the fungus Colletotrichum sp. The attack became severe because it was planted in swamp land. An environmentally friendly potential alternative for controlling anthracnose disease is using antagonistic agents from the bacteria Streptomyces sp. Streptomyces sp. bacteria isolated from 4 swamp lands in South Kalimantan, 2 lowland swamps and 2 tidal swamps. The research aims to determine the ability of Streptomyces sp. Swampland isolates inhibit the growth of the fungus Colletotrichum sp. origin of Hiyung chili in vitro. The research used a Completely Randomized Design (CRD) consisting of 16 treatments based on the origin of the isolates, 4 isolates from Sirang Laut, 4 isolates from Puntik, 4 isolates from Gudang Hirang and 4 isolates from Tajau Landung. Streptomyces sp bacteria. Isolated using selective Yeast Malt Agar media. The results of the exploration found 16 isolates of Streptomyces sp. and all isolates were able to inhibit the growth of Colletotrichum sp. with an inhibition percentage above 50%. The highest percentage of inhibition for isolates from Puntik was 96.44% and the lowest percentage for isolates from Sirang Laut was 51.95%. There were 8 isolates that produced clear zones in the inhibition test which was thought to be due to producing antibiotics, 1 isolate was hyperparasitic, and 7 isolates had an overgrowth mechanism.