Quail is one of the poultry commodities that are increasingly popular in Indonesia. This is evident from the growing interest of people in raising quails and the increasing number of individuals consuming quail products, including eggs and meat. Quail farming has the advantage of being easy to maintain, but there are also several crucial aspects that must be considered to prevent quails from contracting diseases or outbreaks. This can be achieved by implementing biosecurity measures, which are essential factors in managing a farm. The research aims to measure the level of knowledge among farmers, the implementation of biosecurity, and the correlation between knowledge and biosecurity application in Quail Farms in the cities of Kediri and Kediri Regency, East Java Province. The goal is to enhance knowledge about biosecurity in quail farms in the mentioned areas and serve as a reference for further research and application. The research design used is observational (survey) on quail farms in Kediri City, utilizing a questionnaire in the form of a Google form. The collected data is then recorded and tabulated using MS Excel, and subsequently explained descriptively and qualitatively, presented in tables or graphs. The survey and observation results of quail farmers in Kediri City, East Java, show the following average outcomes: 86.5% have good knowledge, 7.5% have sufficient knowledge, and 6% have poor knowledge. Regarding the implementation of biosecurity, 30% were rated as good, 49% as sufficient, and 21% as poor. In conclusion, the knowledge of quail farmers in Kediri City and Kediri Regency, East Java Province, is considered good. However, there is still room for improvement in the implementation of biosecurity measures, as many farmers do not adhere to proper biosecurity management systems.