Bali cattle is one of Indonesia's original germplasm as a producer of the best quality meat compared to other local cattle, so it is important to continue to increase production. One of the efforts to increase the production of Bali cattle is to know the number of bacteria as an indicator that affects the digestive process of feed in different geographical areas. The purpose of this study was to determine the ALTB in the reticulum, omasum, and abomasum of Bali cattle based on geographical location and to determine differences in the ALTB in the reticulum, omasum, and abomasum of Bali cattle in the lowlands and highlands. Sampling in the form of contents of the reticulum, omasum, and abomasum of Bali cattle was carried out at the slaughterhouse. This study used a sample of 32 samples. The method used is the cultivation of bacteria on Nutrient Agar (NA) media with the pouring method. The data obtained are tabulated in the form of Average (Mean) ± Standard Deviation (SD). Then analyzed with the Independent Sample T-test to determine the significant value. The results showed that the amount of ALTB in the reticulum, omasum, and abomasum of Bali cattle reared in the lowlands were 238x105±37,1 CFU/g, 205x105±56,8 CFU/g, 197x105±34,1 CFU/g, respectively. and highlands, namely 248x105±43,1 CFU/g, 223x105±37,5 CFU/g, 211x105±28,2 CFU/g. The results of the Independent Sample T-test continued, namely the value of ALTB in each organ there was no significant difference (P> 0,05) in the lowlands and highlands. This study concluded that there was no significant difference in ALTB in the reticulum, omasum, and abomasum of Bali cattle reared in the highlands and the lowlands. Henceforth, it is necessary to research the characteristics of bacteria in the reticulum, omasum, and abomasum of Bali cattle.