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zulham zulham
Departemen Histologi, Fakultas Kedokteran, Universitas Sumatera Utara
Social Sciences

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The Effect Of Streptomycin And Clindamycin On Dnase I Activity On The Quality Of DNA Of Salivary Origin Hamimatur Rohmah; zulham zulham
Journal Of Social Science (JoSS) Vol 2 No 4 (2023): JOSS : Journal of Social Science
Publisher : Al-Makki Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57185/joss.v2i4.66

Abstract

Deoxyribonucleic acid (DNA) contains genetic material with all an individual’s information. Saliva has the potential to be a good source of human DNA. When compared to blood sampling, saliva is easier to collect and the collection process is non-invasive. However, human DNA collected from saliva has the potential to be degraded due to deoxyribonuclease (DNase) activity in saliva. DNase activity can be inhibited by antibiotics, such as aminoglycoside antibiotics and lincomycin derivatives. This study aimed to determine the effect of streptomycin and clindamycin on DNAse I activity against the quality of human genomic DNA from saliva. The sampling technique was that samples were collected from 9 subjects with each subject flowing into a saliva pot. Before saliva collection, subjects were asked to rinse their mouth with a solution of chlorhexidine gargle (Listerin™) for 30 seconds, then the samples were divided into four groups. The average concentration of DNA extracted from the spin-column method was 32.91 g/mL (7.10-99.45 g/mL) while the average purity was 1.813 g/mL (1.639-2.043 g/mL). With clindamycin treatment, PCR was able to amplify the human NOTCH2 gene (~704 bp) while various concentrations of streptomycin produced multiple bands of ~100 bp. The concentration of 3.2 mM clindamycin effectively inhibits DNAse I activity and can amplify the human NOTCH2 gene while streptomycin cannot protect.
Effect Of Gentamicin And Neomycin+Bacitracin On Deoxyribonuclease Activity I On The Quality Of Human DNA Of Salivary Origin Siti Chairani; Zulham Zulham
Journal Of Social Science (JoSS) Vol 2 No 4 (2023): JOSS : Journal of Social Science
Publisher : Al-Makki Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57185/joss.v2i4.69

Abstract

Deoxyribonucleic acid (DNA) can be found in saliva and various other sources. Saliva contains epithelial cells and leukocytes that are released into the oral cavity. Cells in saliva are potential sources of DNA for diagnostic purposes. DNA in saliva can be degraded due to the activity of deoxyribonuclease (DNase), an enzyme that catalyzes the hydrolysis of DNA. DNase I is expressed in exocrine cells while DNase II is expressed in macrophages. Inhibition of DNase activity will preserve DNA. Besides being able to kill bacteria, aminoglycosides (i.e. gentamicin and neomycin) can inhibit DNase. Saliva was collected from 8 subjects. Furthermore, saliva samples from each subject were divided into 4 groups, namely negative control (K1), positive control (K2), gentamicin (K3), and neomycin + bacitracin (K4). DNA was extracted from saliva using the spin column method. DNA from groups K3 and K4 were added to 1 mg/mL gentamicin and 20 mM neomycin + bacitracin, respectively, before adding 2.5 g/mL DNase I (DNA degradation assay). The quality of human genomic DNA from saliva was determined by the presence of the NOTCH2 gene amplicons (~704 bp). DNA was separated by 1% agarose gel electrophoresis and recorded using the gel documentation system. The DNA extracted from saliva using the spin column method had an average concentration of 26.49 + 30.70 ng/mL and an average purity of 1.819 + 0.122. The administration of distilled water as a negative control in the DNA degradation assay showed that no DNA was digested.
Co-Authors Hamimatur Rohmah Siti Chairani