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All Journal Media Karya Kesehatan Jurnal Pelayanan dan Pengabdian Masyarakat (Pamas) Eduvest - Journal of Universal Studies
Dwi wahyu indrawati
Universitas Muhamadiyah Sidoarjo, Indonesia
Aerospace Engineering Computer Science & IT Decision Sciences, Operations Research & Management Environmental Science Health Professions Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences

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Viabilitas Fibroblast Freeze – Dried Amniotic And Fresh Amniotic In BHK21 Cell indrawati, Dwi wahyu; Prahasanti, Chiquita; Setyawatie, Ernie Maduratna
Eduvest - Journal of Universal Studies Vol. 4 No. 6 (2024): Journal Eduvest - Journal of Universal Studies
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/eduvest.v4i6.1455

Abstract

Amniotic membrane is the inner most lining of the human placenta that is normally discanded after parturition. The membrane has numerous growth factors, proteins and stem cell reserves that help in accelerating wound healing with regeneration of the lost tissues. The preserved human amniotic membrane is a novel tissue engineerrined biomaterial that is recently trial in field of medicine dentistry to regenerate the lost tissues and accelerate repair. This review paper unfolds the inherent structure, properties, mechanisms and the application of this neglected tissue that makes it a potential for regeneration especially in the field of oral and periodontal surgeries. This research has been conducted to measure the difference of viabilitas fibroblast concentration between fresh amniotic and freeze – died amniotic membranes. Experimental three group test design was exployed with amniotic membrane. Amniotic membrane was divided into two parts. The first parts was without preservation amniotic, the test with BHK21 cell for control group, the second part was with reservation freeze –dried amniotic and the last experiment was with preservation fresh amniotic membrane. The result experiment are viabilitas fibroblast freeze – dried amniotic more than fresh amniotic membrane.
Pineapple Prebiotics for Osteoblast Viability and Periodontitis Antibacterial Therapy Indrawati, Dwi Wahyu; Mariyati, Lely Ika; Mukti, Ria Andriani; Zakyzein, Mochammad Alfahrizhy
Media Karya Kesehatan Vol 8, No 2 (2025): Media Karya Kesehatan
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/mkk.v8i2.65302

Abstract

Periodontitis is a chronic inflammatory disease that progressively damages periodontal supporting tissues as a result of colonization by pathogenic bacteria, especially Porphyromonas gingivalis. This condition is characterized by gingival inflammation, loss of periodontal ligament, and resorption of alveolar bone. Conventional therapy through scaling, root planing, and systemic antibiotics is frequently limited by bacterial resistance, relapse, and potential adverse effects. Therefore, alternative natural agents that are safe and able to support bone regeneration are highly required. This study aimed to evaluate the antibacterial activity as well as the biological effect of pineapple (Ananas comosus) prebiotic extract on osteoblast viability. An in vitro experimental design was applied using disc diffusion assays to measure inhibition zones against P. gingivalis and MTT assays to evaluate osteoblast viability. Fermented extracts at concentrations of 10%, 20%, and 30% were tested, with metronidazole as a positive control. Results showed concentration-dependent increases in inhibition zones, namely 7.9 mm (10%), 10.42 mm (20%), and 13.22 mm (30%). MTT assays demonstrated enhanced osteoblast viability at 10% (0.534) and 20% (0.600), whereas viability decreased at 30% (0.551), suggesting possible cytotoxicity at higher doses. ANOVA indicated significant differences among groups (p < 0.05). In conclusion, pineapple prebiotic extract exhibits dual therapeutic potential: antibacterial activity against P. gingivalis and stimulation of osteoblast viability, supporting its development as a promising adjuvant therapy for periodontitis with infection control and alveolar bone regeneration benefits. Keywords: Antibacterial, bromelain, pineapple, osteoblast, periodontitis.
Biopori: Solusi Sampah Organik Untuk Kesuburan Tanah di Taman Toga Desa Sentul, Kec. Purwodadi, Kab. Pasuruan Indrawati, Dwi Wahyu; Aprilia, Erika Putri; Anugraheni, Saskia Dwi; Firdaus, Alfiadny
Jurnal Pelayanan dan Pengabdian Masyarakat (Pamas) Vol 10, No 1 (2026): Jurnal Pelayanan dan Pengabdian Masyarakat (PAMAS)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM Universitas Respati Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52643/pamas.v10i1.5871

Abstract

Sentul Village, Purwodadi District, Pasuruan Regency, has approximately 3,050 residents, with low household organic waste management practices. Improper waste management can increase environmental pollution risks and reduce the productivity of home gardens. This community service activity aimed to improve residents’ knowledge and skills in managing organic waste through the construction of biopore holes in the TOGA (Family Medicinal Plants) Garden. The activity was conducted from February 1 to 11, 2025, involving 50 housewives who collectively constructed 25 biopore holes as a natural composting medium. Evaluation was carried out by measuring the volume of organic waste before and after the activity, as well as pre–post tests using questionnaires to assess participants’ knowledge improvement. The results showed that the utilization of 25 biopore holes reduced the volume of household organic waste sent to the landfill by 30% and increased the average participants’ knowledge score from 62% to 85%. Furthermore, the compost produced enhanced the fertility of the TOGA Garden soil, indicated by improved growth of planted seedlings. In conclusion, the biopore method is an effective and sustainable solution for reducing household organic waste, increasing community knowledge, and improving environmental quality. This approach can be replicated more widely in other communities
Co-Authors Anugraheni, Saskia Dwi Aprilia, Erika Putri Chiquita Prahasanti Firdaus, Alfiadny Lely Ika Mariyati Mukti, Ria Andriani Setyawatie, Ernie Maduratna Zakyzein, Mochammad Alfahrizhy