The background of this research arises from the problem of lack of knowledge about the content of teaching based on the Book of Mark or formal Christian religious education among Gereja Kristen Alkitab Indonesia Sunday school teachers in Central Java. The application of the Teaching Content based on the Book of Mark requires teaching methods based on the Book of Mark that are also effective, creative, and relevant for use today. This type of research is quantitative research with a descriptive analysis approach. Data collection techniques were carried out by observation, interviews, and questionnaires. The sample in this study amounted to 110 respondents with a sampling technique based on Simple Random Sampling. Data analysis uses applications and program formulas known as Statistical Product and Service Solutions (SPSS). The results of this study indicate that there is an influence of teaching content based on the Book of Mark (X1) on the character building of Gereja Kristen Alkitab Indonesia Sunday school children in Central Java with R Square of 0.275, there is an influence of teaching methods based on the Book of Mark (X2) on the character building of Gereja Kristen Alkitab Indonesia Sunday school children in Central Java, with R Squqre of 0.458, and proven to have a significant influence of teaching content based on the Book of Mark (X1) and teaching methods based on the Book of Mark (X2) together on the character building of Gereja Kristen Alkitab Indonesia Sunday school children in Central Java (Y) with adjusted R Square 0.452. Shows the effect of variables X1 and X2 on Y is 0.452 or 45.2% so that the less 54.8% is influenced by factors outside this study.