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Titiek Sumarawati
Departemen Ilmu Biomedik, Fakultas Kedokteran, Universitas Islam Sultan Agung, Semarang, Indonesia
Environmental Science Health Professions Immunology & microbiology Public Health

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Effect of Pomegranate Extract Topical Cream (Punica granatum) on SOD and TNF-α Levels in Wistar Rats Excision Wound Model Retta Adhimarta Atmaja; Agung Putra; Titiek Sumarawati
Gema Lingkungan Kesehatan Vol. 22 No. 2 (2024): Gema Lingkungan Kesehatan
Publisher : Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/gelinkes.v22i2.137

Abstract

Wounds cause damage or loss of skin tissue components. The body will respond and trigger the wound healing process. Neutrophils will trigger the pro-inflammatory mediator, Tumor Necrosis Factor alpha (TNF-α) which is useful for starting inflammation. Providing exogenous antioxidants, such as those found in pomegranates, is very important. Pomegranate extract contains antioxidants such as flavonoids, alkaloids, tannins, triterpenes and saponins which have the potential to be a new treatment for inflammation caused by various skin disorders. Pomegranate extract significantly improves the wound healing process. However, an in-depth analysis of pomegranate extract cream which can accelerate the healing of excision wounds and influence SOD and TNF-α levels needs to be carried out, so that the results of this research can be a new alternative that supports topical wound treatment. An in-vivo experimental study was conducted with 48 Wistar rats, divided into 12 groups. The analysis was performed in two stages: six groups were analyzed after 3 days of treatment, and the remaining six groups were analyzed after 7 days of treatment. Data were tested using one-way ANOVA. Pomegranate extract cream reduced TNF-α levels, as shown by the One-Way ANOVA test (p=0.001, p<0.05). On day 3 after treatment, pomegranate extract cream had the same effect as the bioplacenton group. There was no significant difference between groups in SOD levels on day 3 after treatment, as indicated by the One-Way ANOVA test (p=0.565, p>0.05). However, on day 7 after treatment, there was a significant difference in SOD levels between groups, as shown by the One-Way ANOVA test (p=0.037, p<0.05). Pomegranate extract cream reduces TNF-α levels to the same extent as bioplacenton on day 3 after treatment and increases SOD levels in excision wounds on day 7 after treatment.
The Effect of Habbatussauda (Nigella sativa) on TNF-α and IL-6 Levels in Wistar Rats Induced High Fat Diet Yenyen Sulistio Iriana Karyani; Agung Putra; Titiek Sumarawati
Gema Lingkungan Kesehatan Vol. 22 No. 2 (2024): Gema Lingkungan Kesehatan
Publisher : Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/gelinkes.v22i2.174

Abstract

Habbatussauda (Nigella sativa) contains the active substance thymoquinone which has antihypercholesterolemic, antioxidant, and anti-inflammatory effects. Administration of Habbatussauda oil can improve TNF-α and IL-6 levels in hypercholesterolemia conditions. The research aimed to determine the effect of giving habbatussauda on levels of TNF-α and IL-6 in rat fed a high-fat diet. Post-test-only control group design experimental research for 45 days. A sample of 30 Wistar rats aged 8 weeks, weighing 150-200 grams and adapted for 7 days, were randomly divided into 5 groups, namely: normal control group (K1), negative control group given a high-fat diet (K2), treatment group given habbatussauda 0.001 mL/gBB (K3), treatment group given habbatussauda 0.002 mL/gBB (K4), treatment group given habbatussauda 0.004 mL/gBB (K5) treatment was given for the last 2 weeks. TNF-α and IL-6 levels were measured by the ELISA method. Data were analyzed using One Way Anova then continued with the Post Hoc LSD. The mean ± SD of TNF-α levels in groups K1, K2, K3, K4 and K5 were 83.36 ± 23.73, 246.47 ± 43.21, 155.83 ± 36.38, 171.80 ± 22.50, and 195.77 ± 47.67 pg/mL, respectively. In IL-6 groups K1, K2, K3, K4 and K5 were 33.83 ± 8.55, 115.11 ± 16.51, 58.85 ± 19.71, 76.70 ± 6.03, and 78.05 ± 17.33 pg/mL, respectively. One Way Anova test for TNF-α and IL-6 levels showed significant differences between groups (p = 0.000 and p = 0.000). In the post hoc test, TNF-α and IL-6 levels in K3, K4, and K5 were significantly lower than in K2 (p = 0.000). Administration of Habbatussauda can reduce TNF-α and IL-6 levels in rat induced by a high-fat diet.
Topical Antioxidant Potential of Telang Flower Extract (Clitoria ternatea L.) on IL-6 and VEGF Regulation in UV-B Exposure: An In Vivo Experimental Study Retno Adaninggar; Titiek Sumarawati; Chodidjah
Gema Lingkungan Kesehatan Vol. 23 No. 2 (2025): Gema Lingkungan Kesehatan
Publisher : Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/gelinkes.v23i2.204

Abstract

UVB radiation can penetrate the epidermis and can induce DNA damage in skin cells by increasing ROS concentrations. Apart from that, UVB radiation also increases melanocyte proliferation which can cause melasma. Skin damage due to UV exposure is caused by the generation of  ROS and various inflammatory factors. Telang flowers contain Glutathione, which is one of the main antioxidants in the body, which has a skin depigmentation function. One of the physiological effects of glutathione is to inhibit melanogenesis by suppressing tyrosinase activity. Experimental research with post test control group. Groups KN, P1 and P2 were each exposed to UVB at 302 nm with a MED of 160 mJ/cm2, while group K0 was a healthy group. P1 was given 5% butterfly pea flower gel and P2 was given 10% gel every day for 14 days, while KN received base gel. On the 21st day, the tissue was analyzed for IL-6 and VEGF level using ELISA. There was a significant difference in the mean levels of IL-6 between the four groups, with the One Way Anova test p = 0.000 (p <0.05). IL-6 levels decreased with increasing doses (P1=32.49±1.02, K4=27.82 ± 0.74) compared to the control (KN=61.89 ± 0.69, while K0=25.30 ± 0.55). Furthermore, the mean VEGF levels also had a significant difference between the four groups with the Kruskal Wallis test p = 0.000 (p <0.05) VEGF levels in the control group (KN = 37.44 ± 2.43) and followed by the healthy group (K0 = 23.47 ± 0.99), then in the P1 treatment group (K3 = 30.96 ± 0.57) were higher than the P2 treatment group (K4 = 27.00 ± 1.55). Administration of butterfly pea flower gel can reduce the level of the IL-6 gene and increase the level of the VEGF gene in the skin tissue of mice models of UVB light-induced hyperpigmentation.
Co-Authors Agung Putra Chodidjah Retno Adaninggar Retta Adhimarta Atmaja Yenyen Sulistio Iriana Karyani