Betris Sanda Sinambela
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PERTUMBUHAN SUBKULTUR BUAH NAGA DENGAN PENAMBAHAN NAA DAN BAP Betris Sanda Sinambela; Asnawati Asnawati; Nur Arifin
Agros Journal of Agriculture Science Vol 26, No 2 (2024): Edisi Juli
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v26i2.4703

Abstract

known as "dragon fruit" which is widely cultivated in various countries in Asia. The high demand for dragon fruit makes the availability of seeds for dragon fruit development difficult. The in-vitro technique is a suitable alternative to provide large quantities of seed in a short time. Media and growth regulators are the determining factors for explant growth. The combination of NAA and BAP is suitable for dragon fruit, which is useful for triggering cell division in the tissue made by explants and triggering shoot growth, root formation, stem elongation and root elongation. The purpose of the study was to obtain the best interaction of Naphtaleine Acetic Acid and Benzyl Amino Purine concentrations for dragon fruit subculture growth. This study used a Factorial Completely Randomized Design (CRD) consisting of two factors, namely Naphtaleine Acetic Acid concentration (0.2 ppm, 0.4 ppm, 0.6 ppm, 0.8 ppm) and Benzyl Amino Purine concentration (1 ppm, 2 ppm, 3 ppm 4 ppm) with each consisting of 4 concentration levels, resulting in 16 treatment combinations. The treatment was repeated 3 times and each treatment unit consisted of 4 samples. The variables observed in this study were the percentage of sprouted explants (%), the time of the first shoot (week after planting), the number of shoots (buds), the time of the first root appearance (week after planting) and the number of roots (strands). The results showed that the interaction of NAA and BAP concentrations did not produce the best interaction concentration effect on dragon fruit explants so that the objectives were not achieved. At a concentration of 0.6 ppm NAA is effective in increasing the number of roots of dragon fruit explants and a concentration of 1 ppm BAP is effective in accelerating the time of root emergence and increasing the number of roots of dragon fruit explants.