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All Journal Jurnal Nukleus Peternakan
I Wayan Suberata
Fakultas Peternakan Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Veterinary

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MOTILITAS DAN VIABILITAS SPERMATOZOA BABI DENGAN HOLDING TIME DAN LAMA THAWING BERBEDA (Sperm motility and viability of boars in different holding and thawing time) Ni Luh Manik Sugiantini; Ni Luh Gde Sumardani; I Wayan Suberata
JURNAL NUKLEUS PETERNAKAN Vol 10 No 2 (2023): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v10i2.11175

Abstract

The motility and viability of sperm are the main factors in determining semen quality. The treatment of semen before and after dilution is a determining factor whether or not the semen is suitable for use in artificial insemination. This study aims to determine the effect of holding time and thawing time on the motility and viability of boars sperm. This research was conducted at the Livestock Reproduction Laboratory, Bukit Jimbaran for 1 month, using a factorial randomized block design The first factor is the holding time which consists of 3 levels: 0 hours (HT0), 2 hours (HT2), and 3 hours (HT3). The second factor is the thawing time which consists of 3 levels: for 2 minutes (T2), 3 minutes (T3), and 5 minutes (T5). There are 9 combinations and 5 groups based on sperm storage time. The observed variables included the motility and viability of boars sperm. The results showed that the HT3 treatment was significantly different (P<0.05) with 22% sperm motility and 41.55% sperm viability. Thawing for 5 minutes (T5) gave the best results with sperm motility 18.50% and sperm viability 38.45%. There was no interaction between holding time and thawing time. The conclusion of this study is that boars sperm shows the best results at holding time of 3 hours with a thawing time of 5 minutes.
KUALITAS SEMEN BEKU SAPI BALI PASCA THAWING DALAM WAKTU BERBEDA (Quality of Bali Bull Frozen Semen Post Thawing in Differents Times) Fafandri .; Ni Luh Gde Sumardani; I Wayan Suberata
JURNAL NUKLEUS PETERNAKAN Vol 10 No 2 (2023): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v10i2.11176

Abstract

The aim of this research was to know quality of bali bull frozen semen post thawing in defferent times. The research was conducted in Livestock Reproduction Laboratory of the Faculty of Animal Husbandry, Udayana University, Bukit Jimbaran, bali Province in one months. The treatment of this research are T 1 (thawing in 15 seconds), T 2 (thawing in 30 seconds), and T3 (thawing in 60 seconds) on temperatur 37oC. The data of research was sperm motility, sperm viability, and sperm abnormality. Result of this research showed that the quality of bali bull frozen semen post thawing in defferent times that 15 second (T 1), 30 second (T 2), and 60 second (T 3) did not significantly (P> 0.05). The best thawing is at T3 treatment with sperm motility 71.00±2.00%, sperm viability 56.07±1.69%, and sperm abnormality 1.97±0.47%. The conclusion of this study is the bali bull frozen semen can be thawed at temperature 37oC in 60 seconds.
Co-Authors Fafandri . Ni Luh Gde Sumardani Ni Luh Manik Sugiantini