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All Journal JURNAL PESISIR DAN LAUT TROPIS
Veibe Warouw
UNSRAT
Earth & Planetary Sciences

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FILOGENI MOLEKULER BAKTERI DARI MEDIA PEMELIHARAAN ROTIFER YANG DIBERI OLAHAN LIMBAH IKAN SEBAGAI SUMBER NUTRISI Herlin S Hubu; Stenly Wullur; Veibe Warouw; Elvy L Ginting; Robert A Bara; Adnan S Wantasen
JURNAL PESISIR DAN LAUT TROPIS Vol. 9 No. 1 (2021): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.1.2021.33574

Abstract

This study aims to identify and construct molecular phylogeny of an isolate bacteria from culture media of rotifer Brachionus rotudiforis supplied with processed fishery waste feed as nutritional source. The use of fish waste-based food for rotifer showed positive effects on growth and nutrient content of the rotifers. Genomic DNA of the isolate bacteria BRLI- 01 was extracted and the 16S rRNA gene was amplified using primers (8F and 1492F) and further sequenced using Sanger sequence technique. The 16S rRNA gene was analysed using SeqScanner® and MEGA® followed with BLAST (Basic Local Alignment Search Tool) analyses in the NCBI (National Centre for Biotechnology Information). Amplification result of 16S rRNA gene bacteria s NCBI site as a reference for identification and phylogeny of bacterial species. BRLI-01 was successfully cultured on rotifer rearing media. The results of the 16S rRNA gene amplification of the isolate bacteria showed a DNA band with a length of 1400 bp. The BLAST result on the NCBI showed that the isolate bacteria BRLI-01 had a percent identity (98.46%) and is in the same phylogony branching position with Vibrio rotiferianus Keywords: Rotifers, Bacteria, Fish waste, 16S rRNA Genes, Phylogeny identification
BIOINDEKS ZOOPLANKTON DI PERAIRAN PULAU BUNAKEN Kezia Keren Erika Tampi; Joice R.T.S.L Rimper; Veibe Warouw
JURNAL PESISIR DAN LAUT TROPIS Vol. 9 No. 1 (2021): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.9.1.2021.33624

Abstract

The city of Manado has several islands which are strategic places with the development of marine potential. One that is so potential is the waters of Bunaken Island and the forms of activity in Bunaken Island waters vary widely. The purpose of this study is to determine the abundance value and biological index and to analyze the condition of the waters in the waters and to see the comparison at each research station. The results of zooplankton identification consisted of 7 genera including all research stations. The results of the analysis of the highest abundance data were at the third station (8.4822 ind / l) and the lowest abundance value was at the first station (7.4220 ind / l). The results of the biological index analysis obtained are as follows: the average diversity value at three stations is 1.08, the uniformity is 0.98 and the dominance is 0.20. The results of the Morisita index for each station are: stations. Which means the value of the distribution pattern is three groups, and the others are uniform in each research genus, the overall distribution is uniform.Keywords:  Zooplankton, Bunaken Island waters, Abundance, Bioindex
Co-Authors Adnan S Wantasen Elvy L Ginting Herlin S Hubu Joice R.T.S.L Rimper Kezia Keren Erika Tampi Robert A Bara Stenly Wullur