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Suciatmih, Suciatmih
Research Center for Biology-Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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ISOLASI, IDENTIFIKASI DAN EVALUASI ANTAGONISME TERHADAP Fusarium oxysporum f.sp. cubense (Foc) SECARA IN VITRO DARI JAMUR ENDOFIT TANAMAN PISANG [Isolation, Identification and Evaluation of Antagonism to Fusarium oxysporum f.sp. cubense (Foc) Under in Vitro Conditions from Endophytic Fungi of Musa sp.] Suciatmih, Suciatmih; Hidayat, I; Sulistiyani, TR
BERITA BIOLOGI Vol 13, No 1 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.11 KB) | DOI: 10.14203/beritabiologi.v13i1.656

Abstract

Isolation of endophytic fungi was done to find alternative microorganisms as antifungal agent against Fusarium oxysporum f.sp. cubense (Foc), causing panama disease (fusarium wilt) on Musa spp. The research objectives were 1) to isolate and identify endophytic fungi colonize cavendish (AAA) and rejang (AA) bananas growing on PT Natural Tropical Fruit (NTF) Plantation in East Lampung; and 2) to evaluate for their antagonistic activity against Foc under in vitro conditions. The results indicated 46 isolates of endophytic fungi that were isolated from leaves, saplings and petioles of cavendish banana; and leaves and petioles of rejang banana. The isolated endophytic fungi belonging to the group of Colletotrichum, Fusarium, Guignardia, Penicillium, Talaromyces and non sporulating endophytic fungi that were classified as unidentified isolate. Fourteen isolates (30.43 %) of those 46 fungi isolates tested, showed varying degree of antagonism to Foc. The best three isolates that have strong antagonistic activities were Talaromyces sp. 27-4 (M), unidentified 23-3/11 (B) and unidentified 26-5/K (L). Fusarium oxysporum 22-3/F (I), Penicillium sp. 20-2/H (J), Talaromyces sp. 27-4 (M) and unidentified 21-1/6 (A) inhibited the growth of Foc by producing both volatile and unvolatile metabolites. The strongest inhibition (53.17 %) by volatile inhibitory subtance was produced by F. oxysporum 22-3/F (I), while the strongest inhibition (65.98 %)by unvolatile inhibitory subtance was produced by Penicillium sp. 20-2/H (J).
KEANEKARAGAMAN DAN DAYA DEGRADASI SELULOSA JAMUR TANAH DIHUTAN BEKAS TERBAKAR WANARISET-SEMBOJA, KALIMANTAN TIMUR Suciatmih, Suciatmih
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (846.332 KB) | DOI: 10.14203/beritabiologi.v9i2.2026

Abstract

In order to know the effect of isolation method on the occurrence and capability of soil fungi to degrade cellulose,a study was conducted in postburning forest in Wanariset-Semboja, East Kalimantan. Soil fungi were isolated using three isolation methods:incubation at 45° C, treatment with 50 % ethanol for 15 minutes, and heat treatment at 70° C for 15 minutes.Plates for heat incubation and for other methods were incubated at 45° C and 27° C for three days, respectively. Cellulose degradation test of isolated fungi was examined using Carboxy Methyl Cellulose (CMC) media. Results showed that isolation method affected diversity and population of soil fungi. Heat treatment at 70° C for 15 minutes appeared to have highest diversity and population of soil fungi. Eupenicillium Javanicum var javanicum (van Veyma) Stolk & Scott, Talaromyces byssoclamydoides Stolk & Samson, T. flavus(Klocker) Stolk & Samson, T. stipitatus C.R. Benjamin, and Penicillium argillaceum Stolk et al. were dominant in postburning forest in Wanariset-Semboja, East Kalimantan. Twenty-one isolated fungi degraded cellulose.
PENGUJIAN SURVIVAL JAMUR YANG DIPRESERVASI DALAM AIR DAN PARAFIN CAIR Suciatmih, Suciatmih; Rachmat, Rachmat
BERITA BIOLOGI Vol 7, No 5 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.754 KB) | DOI: 10.14203/beritabiologi.v7i5.874

Abstract

A study has been conducted for survival testing of 7 and 7-16 years-preserved fungi in water and liquid-paraffin respectively.Survival testing was done through replanting the preserved fungi in new PDA slant media.The growth observation of fungi was carried out for two weeks long.Each collection number was tested with two replications. Thirteen collection numbers of fungi (Alternaria spp.,Aspergillus spp.,Fusarium spp.,Penicillium spp.,and Trichoderma spp.,)which were preserved for 7 and 8 years long in water and liquid-paraffin respectively were growing again, and the rate of survival were 100%. The survival of the fungi(species and sum of sample were different) which were preserved in liquid-paraffin for 7, 8, 9 and 16 years long were 38,5, 81,5,53,6, and 64,7% respectively.
TEST OF LIGNIN AND CELLULOSE DECOMPOSITION AND PHOSPHATE SOLUBILIZATION BY SOIL FUNGI OF GUNUNG HALIMUN Suciatmih, Suciatmih
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.093 KB) | DOI: 10.14203/beritabiologi.v5i6.1074

Abstract

In order to know the capability of lignin and cellulose degradation and phosphate solubilization by soil fungi of Gunung Halimun National Park, a study was carried out to qualitatively analyse its physiological properties. Out of 35 soil fungi tested, 1 species that belonged to Basidiomycetes degraded lignin, 32 species degraded cellulose, and 31 species dissolved inorganic phosphate.The presence of soil fungi that degraded cellulose and dissolved phosphate could be used as a candidate for biotechnology application as well as to increase soil fertility.
ARBUSCULAR MYCORRHIZAL FUNGI AT DIFFERENT ECOSYSTEMS OF GUNUNG HALIMUN NATIONAL PARK*[Jamur Mikoriza Arbuskula pada Ekosistem Berbeda di Taman Nasional Gunung Halimun] Suciatmih, Suciatmih; Kramadibrata, K.
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.417 KB) | DOI: 10.14203/beritabiologi.v6i1.1182

Abstract

Telah berhasil dikoleksi tujuh jenis jamur mikoriza arbuskula (MA) dari tiga macam ekosistem (masing-masing dari satu tempat yang tidak terganggu dan dua tempat yang terganggu) di Ciptarasa, Taman Nasional Gunung Halimun (TNGH).Jamur-jamur tersebut adalah Acaulosporafoveata,A. morrowiae, Glomus cf.aggregation,G.etunicatum, G cf. glomerulatum,G.cfmultisubstensum and Scutellosspora projecturata. Keanekaragaman jamur MA ada kecendeningan menurun oleh adanya perusakan ekosistem.Tempat yang rusak pada ketinggian 500 m dan 700 m dpi,mempunyai keanekaragaman jamur lebih rendah, yaitu masing-masing satu dan dua jenis dari pada tempat yang tidak rusak pada ketinggian 1000 m dpi (6 jenis).
Co-Authors Hidayat, I Kramadibrata, K. RACHMAT RACHMAT Sulistiyani, TR