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Hutabarat, Dameria
Research Center for Biology-Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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REGENERASI TANAMAN DARI JARINGAN KOTILEDON +RUAS BENIH KEDELAI, {Glycine max L., KULTIVAR WILIS) DENGAN RADIASI SINAR GAMMA [Plant Regeneration from Cotyledons of Mature Soybean ( Glycine max L. Wilis Cultivar Using Gamma Rays] Hutabarat, Dameria; Ratma, Rivaie
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (412.161 KB) | DOI: 10.14203/beritabiologi.v4i5.1245

Abstract

Soybean Wilis cultivar was efficiently regenerated in vitro via somatic embryogenesis. Cotyledonary explants were excised from mature geminating seeds. Seeds were germinated on agar solution and on B5 medium enriched with 5 ppm BA, 0,25 pom IBA and 500 ppm casein hydrolyzate. Cotyledonary nodes from both germinating seeds were excised and cultured on B5 medium enriched with 5 ppm BA, 0,25 ppm IBA and 500 ppm casein hydrolyzate. Age of seedlings had a remarkable influence on shoot regeneration. Cotyledon from seeds germinated on agar solution with light gave better result in shoot regeneration compare with those germinated in darkness. The highest number of regenerants per explants (5 shoots) was produced by cotyledon from seeds germinated on 65 medium enriched with 5 PPM BA, 0,25 PPM IBA and 500 PPM casein hydrolyzate in darkness. The seeds of soybean were exposed to gamma-rays doses 10 Gy then germinated on S5 medium enriched with 5 PPM BA,0,25 PPM IBA and 500 ppmcase/n hydrolyzate did not improve the number of plant regeneration.Only 5-day-old seedlings from seeds were exposed to gamma-rays dose 30 Gy could improve the number of shoot regeneration, one of the cotyledonary node treated produced 21 regeneration shoots.
PENGARUH SINAR GAMMA PADA BENIH MANGGIS (Gardnia mangostana L.) [Effects of Gamma Rays on Mangosteen (Garcinia mangostana L.) Seeds] Hutabarat, Dameria
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (306.652 KB) | DOI: 10.14203/beritabiologi.v5i1.1105

Abstract

Mangosteen seeds were irradiated with gamma rays from Co dosages 2.5 Gy, 5 Gy, 7.5 Gy, 10 Gy, 20 Gy, 40 Gy and 60 Gy. The germination rates of irradiated seeds were reduced by an average of 20 % at 20 Gy, 67 % at 40 Gy and no seed germination at 60 Gy.The effects of the treatment on the survival of irradiated seeds were observed.Gamma rays at low dosage (2.5 Gy) was stimulating to seedling growth. The best dosages for inducing variation were around 10 - 20 Gy;variation induced by the treatment included total leaves number and seedling height.
Co-Authors Ratma, Rivaie