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Sumerta, I Nyoman
Research Center for Biology-Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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DIVERSITY OF XYLOSE ASSIMILATING YEAST FROM THE ISLAND OF ENGGANO, SUMATERA, INDONESIA [Keragaman Khamir Pengguna Xilose yang Diisolasi dari Pulau Enggano, Sumatera, Indonesia] Kanti, Atit; Sumerta, I Nyoman
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3160.618 KB) | DOI: 10.14203/beritabiologi.v15i3.2215

Abstract

Naturally occurring yeasts (wild type) are important genetic resources for many industrial interests. Though biodiversity exploration of Enggano has been done since 1944, but there is no report for yeast diversity from this island. Due to its industrial interest, xylose fermenting yeast is target for many scientists. This present study aims to isolate xylose assimilating yeast from island of Enggano. The samples were collected from varying sources included soil, leaf litter, decay wood, fruit and sediment. Xylose enrichment culture technique was performed to enrich xylose fermenting yeast. While for the isolation of yeast several methods which include dilution, ballistopore falling, direct isolation, membrane filtration were done. Of 200 isolates, 76 strains (38 %) were xylose assimilating yeast which mostly belong to Candida which include C. insectorum, C. tropicalis, C. boidinii, C. pseudolambica, C. yuanshanica, C. silvae, Cyberlindnera saturnus, Williopsis saturnus and Sporobolomyces poonsookiae. One strain Candida sp. that was isolated from soil could be assigned as a candidate of novel species on base on its morphological and biochemical characteristics along with analyses of gene sequence from D1/D2 domain. This study confirms that yeast survey is very important to obtain new genetic resources for industrial interest as well as for taxonomic study.
KEANEKARAGAMAN KHAMIR YANG DIISOLASI DARI SUMBER DAYA ALAM PULAU ENGGANO, BENGKULU DAN POTENSINYA SEBAGAI PENDEGRADASI SELULOSA [Diversity of Yeasts Isolated From Natural Resources of Enggano Island, Bengkulu and Its Cellulolytic Potency] Sumerta, I Nyoman; Kanti, Atit
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i3.2220

Abstract

The present study revealed the occurrence and diversity of yeasts from natural resources of Enggano Island. The yeasts were isolated from soil, sediment, leaf, leaf litter, fruit, and flower. Isolation was performed using direct plating, membrane filtration, ballistospore-falling and enrichment culture using glucose, xylose and xylan as carbon source. After isolating, yeasts were screened for its cellulolytic potency. The occurrence of yeasts in different isolation technique are clearly found. Most of 87 yeasts isolated, abundant in leaf, soil, and sediment sample. The total yeasts isolated were classified into 32 species based on D1/D2 LSU 26S rDNA sequences analysis which genera of Candida were predominant (33%). The predominant yeast species were identified as Candida tropicalis (16,1%), Cyberlindnera saturnus (16,1%), and Rhodosporidium paludigenum (11,5%). Screening on carboxymethyl cellulose medium, there are 43 isolates within 22 yeasts species have cellulolytic activity. Four species of Basidiomycetous have high activity which includes Pseudozyma antartica Y15Eg001; Pseudozyma hubeiensis Y15Eg015; Anthracocystis chrysopogonis Y15Eg072; Cryptococcus laurentii Y15Eg017 and two from Ascomycetous are Sarocladium bactrocephalum Y15Eg226, Y15Eg227. Some of cellulolytic yeast isolates are indicated as new species candidate and required to reconfirm in another conserve regions to ensure its taxonomic position. Various yeasts isolated from Enggano Island with its cellulolytic potency should contribute to scientific information regarding microbial genetic resources of outer islands of Indonesia.
Co-Authors Atit Kanti