Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Pelita Eksakta 3BIO: Journal of Biological Science, Technology and Management
Dwi Hilda Putri
Department of Biology, Faculty of Mathematics and Sciences, Universitas Negeri Padang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Mathematics Neuroscience Physics Other

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
The Primer Design and Optimization of Annealing Temperature for Analysis of Glutathione Reductase Gene Expression in Rice (Oryza sativa L.) Annisa Khaira; Afifatul Achyar; Zulyusri Zulyusri; Yusni Atifah; Dwi Hilda Putri; Violita Violita
3BIO: Journal of Biological Science, Technology and Management Vol. 5 No. 1 (2023)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2023.5.1.3

Abstract

Glutathione Reductase (GR) belongs to the NADPH-dependent flavoprotein oxidoreductase family and is found in both prokaryotes and eukaryotes. The GR gene is considered to play a key role in the elimination of oxidative reaction products by looking at the level of gene expression of GR rice in dealing with  drought stress using qPCR. One of the important steps to develop a specific, effective and efficient qPCR is the primer design. Several studies analyzing GR gene expression in rice have also designed primers. However, the primer still lacks an ideal characteristic of primer, as it still has a secondary structure. This studies aims to design rice GR specific primers and optimize the annealing temperature for GR gene expression analysis on rice. Primers were designed using the  Primer3 and Geneious Prime and checked for specificity using the Primer-BLAST tool. The selected primer pairs were then optimized for annealing  temperature using gradient PCR. The best primer design results were GR-Forward 5’-ACGATTGCAGCCAGTGAAGA-3’ and GR-Reverse 5’-TGCGGCAATACTATCAACATCC-3’, with an amplicon length of 204 bp, primer base lengths of 20 and 22 nucleotides, Tm values of 60°C and 58.9°C, %GC of 50% and 45.5%, respectively. This primer pair had no secondary structure, both hairpin and self dimer. Gradient PCR showed the optimum annealing temperature for this primer pair was 52.2oC so that the primer can be used as a specific primer to analyze  the GR gene expression in rice using qPCR.
Improving Early Awareness of Body Organ Health in Students of State Elementary School 01 Pasar Laban Through Interactive Device-Based Education Fadilaturahmah Fadilaturahmah; Sa’diatul Fuadiyah; Putri Qalbina; Kheniva Diah Anggita; Yusni Atifah; Threo Wanda Marten; Afifatul Achyar; Dwi Hilda Putri; Dezi Handayani; Defni Yannita
Pelita Eksakta Vol 9 No 1 (2026): Pelita Eksakta, Vol. 9, No. 1
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol9-iss1/304

Abstract

The health of elementary school-aged children is a crucial factor that needs attention. However, the low awareness of students, especially at SDN 01 Pasar Laban, regarding the importance of maintaining healthy body organs is a problem that needs to be addressed immediately. One of the causes is the limited interactive learning methods in schools, especially regarding body organ health. Therefore, an innovative approach is needed in health education for students, namely a learning method based on interactive teaching aids "Human Body Anatomy Model". Community service activities were carried out on August 6, 2025. Material on body organ health education was delivered using an interactive lecture method followed by game-based learning using interactive teaching aids. This activity increased students' knowledge by 11.11% regarding the introduction of the structure and function of body organs and the effects of unhealthy snack consumption behavior.
Co-Authors Achyar, Afifatul Afifatul Achyar Annisa Khaira Atifah, Yusni Defni Yannita Dezi Handayani Fadilaturahmah Fadilaturahmah Kheniva Diah Anggita Putri Qalbina Sa’diatul Fuadiyah Threo Wanda Marten Violita Violita Yusni Atifah Zulyusri Zulyusri