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All Journal Indonesian Aquaculture Journal
Traifalgar, Rex Ferdinand Mallare
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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THE GROWTH KINETICS AND TOTAL LIPID CONTENT OF Thalassiosira sp. (BACILLARIOPHYCEAE) UNDER MIXOTROPHIC CONDITIONS Nodque, Kelee Ira Burgan; Dionela, Cleresa Salido; Huervana, Fredson Hervias; Traifalgar, Rex Ferdinand Mallare
Indonesian Aquaculture Journal Vol 19, No 1 (2024): (June, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.1.2024.1-9

Abstract

Conventional microalgae culture is challenged by issues of light limitation and cell self-shading. This study aims to evaluate the impact of different cultivation modes on the growth and lipid content of Thalassiosira sp. The diatom, Thalassiosira sp., was grown in autotrophic, mixotrophic-suspended, and mixotrophic-biofilm conditions until the stationary phase was reached. After four (4) days of culture, analysis of the cell densities revealed a significant difference between groups, with cell densities of 7.3×105 cells mL-1 for control, 1.1×106 cells mL-1 for mixotrophic-suspended, and 1.9×106 cells mL-1 for mixotrophic-biofilm cultures. Both treatments are significantly higher than the control. However, mixotrophic-biofilm culture achieved the highest cell density among all cultivation modes, 161.81% higher than the control. The specific growth rate (SGR) of Thalassiosira sp. in mixotrophic-biofilm culture was highest among treatments, while the doubling time was significantly highest in the control. Moreover, mixotrophic-biofilm culture attained the highest biomass at 56 mg 100 mL-1. Thalassiosira sp. cultured under mixotrophic-biofilm also recorded the highest lipid content at 9.89%. It is both significantly higher than the control (3.06%) and the mixotrophic-suspended culture (6.15%). The cell density, algal biomass, and lipid content of Thalassiosira sp. under mixotrophic-biofilm culture highlight this culture strategy’s promising potential in improving microalgae growth and lipid content, ridding of light as an indispensable growth factor.
EFFECT OF DIFFERENT SUBSTRATES ON BIOFILM GROWTH AND LIPID CONTENT OF DIATOM Thalassiosira sp. Tabaquirao, Joan Torrento; Dionela, Cleresa Salido; Huervana, Fredson Hervias; Traifalgar, Rex Ferdinand Mallare
Indonesian Aquaculture Journal Vol 20, No 1 (2025): (June, 2025)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.20.1.2025.97-105

Abstract

Diatoms are valuable as natural feed in aquaculture due to their lipid content and the presence of essential polyunsaturated fatty acids (PUFAs), including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). While traditional suspended cultivation has limitations, attached cultivation offers advantages such as lower water use and improved lipid productivity. This study evaluated the growth, biomass, and lipid content of Thalassiosira sp. grown on polycarbonate (PC) and polyvinyl chloride (PVC) as attachment substrates. The control group was cultured in standard suspended cultivation method.  Test attachments substrates were submerged in sterile seawater enriched with F-medium, and growth was monitored for four days.  At day 4 of the culture, cell density was significantly higher on PC (13.08 × 105 cells mL-1) and PVC (13.01 × 105 cells mL-1) compared to the control (7.93 × 105 cells mL-1). The specific growth rate was also significantly higher on both substrates, exhibiting a doubling time of 0.20 days. Biomass accumulation was highest on PC (27.47 mg 100 mL-1), followed by PVC (26.87 mg 100 mL-1), representing increases of 38.39% and 35.37% over the control (19.85 mg 100 mL-1), respectively. Lipid content was higher in the attached culture system, reaching 8.50% on PC and 7.45% on PVC, corresponding to increases of 167.30% and 134.28% over the control (3.18%). These findings highlight the potential of PC and PVC as effective substrates for biofilm-based cultivation of Thalassiosira sp., demonstrating superior growth, biomass yield, and lipid accumulation compared to the suspended culture method. 
Co-Authors Dionela, Cleresa Salido Huervana, Fredson Hervias Nodque, Kelee Ira Burgan Tabaquirao, Joan Torrento