Benaya Yamin Onesiforus
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Pengaruh Pemanasan Berulang Media Nutrient Agar terhadap Hasil Uji ALT Bakteri Staphylococcus aureus dan Escherichia coli Elisa Rinihapsari; Benaya Yamin Onesiforus; Salsa Aten Riya
Vitamin : Jurnal ilmu Kesehatan Umum Vol. 1 No. 3 (2023): July : Jurnal ilmu Kesehatan Umum
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/vitamin.v1i3.400

Abstract

Nutrient Agar is a universal medium containing agar, meat extract, yeast extract and peptone. NA media is often made in large quantities, stored under sterile conditions, and then reheated when needed. Repeated heating can reduce the number of bacterial colonies that grow because the components that make up the media become damaged. This study aimed to determine the effect of repeated heating of NA (Nutrient Agar) media 4 times on the Total Plate Count (TPC) test results for Staphylococcus aureus and Escherichia coli bacteria. The number of bacteria that grew on the media with varying amounts of heating was calculated, and the results showed that repeated heating 4 times caused a decrease in the number of bacterial colonies that grew on the NA media. The ANOVA test gave a value of p = 0.000 for the two types of bacteria separately, which showed that there is a significant difference between the number of bacteria in varying amounts of media heating. This research concluded that repeated heating of NA (Nutrient Agar) media affects the TPC test results for Staphylococcus aureus and Escherichia coli.
Pengaruh Pemanasan Berulang Media Nutrient Agar Terhadap Hasil Uji Sensitivitas Elisa Rinihapsari; Benaya Yamin Onesiforus; Samuel Marchel Nugroho
Antigen : Jurnal Kesehatan Masyarakat dan Ilmu Gizi Vol. 1 No. 4 (2023): November : Antigen: Jurnal Kesehatan Masyarakat dan Ilmu Gizi
Publisher : LPPM STIKES KESETIAKAWANAN SOSIAL INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/antigen.v1i3.191

Abstract

Nutrient agar media is a universal medium that can be used in bacterial sensitivity tests. Sensitivity testing is used to test the sensitivity of bacteria to a type of antibiotic. This test is instrumental in determining appropriate therapy for patients who experience recurrent or persistent infectious diseases. Nutrient Agar media is usually made in large quantities for time and energy efficiency. The media is used as needed, and the rest is stored 2-8oC in the refrigerator before being used again. This research aims to determine the effect of repeated heating of Nutrient Agar media on the sensitivity test results for Staphylococcus aureus and Escherichia coli bacteria. Tests were carried out on the first, second, third and fourth heating times. The results of statistical tests with Kruskal Wallis showed a sig value of <0.05, meaning there was a difference in sensitivity test results between media heated once, twice, three times, or four times for the two types of bacteria tested.