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All Journal Jurnal Fitopatologi Indonesia Jurnal Agrisa
Hahuly, Mayavira Veronica
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Pathogenicity of Botryodiplodia theobromae on Sandalwood Stems and Its in Vitro Inhibition by Trichoderma spp. Wolagole, Sane; Simamora, Agnes Virginia; Hahuly, Mayavira Veronica
Jurnal Fitopatologi Indonesia Vol 19 No 6 (2023): November 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.6.238-245

Abstract

Penelitian dilaksanakan untuk menentukan patogenisitas Botryodiplodia theobromae dalam menginfeksi batang cendana dan menguji kemampuan 10 isolat Trichoderma spp. dalam menghambat pertumbuhan dua isolat B. theobromae secara in vitro. Uji patogenisitas dilakukan dengan menginokulasi potongan isolat B. theobromae pada batang cendana. Uji antagonis dilakukan menggunakan metode kultur ganda dengan 10 isolat Trichoderma spp. dan dua isolat B. Theobromae sebagai kombinasi perlakuan. Hasil uji patogenisitas membuktikan bahwa B. theobromae menyebabkan gejala penyakit dimulai pada hari ke 30 setelah inokulasi. Hasil uji antagonis menunjukkan bahwa isolat-isolat Trichoderma spp. mampu menghambat pertumbuhan dua isolat B. theobromae melalui antibiosis dan kompetisi. Penghambatan terbaik terhadap B. theobromae Bt01 dicapai oleh T. viride TVJKS (53.20%), sedangkan penghambatan terbaik terhadap B. theobromae Bt02 dicapai oleh T. hamatum THAK (53.26%).
PENGARUH MIKORIZA TERHADAP PENYAKIT LAYU BAKTERI (Ralstonia solanacearum) PADA TANAMAN TOMAT (Licopersicum esculentum) Kolo, Maria Serliana; Hahuly, Mayavira Veronica; Iburuni, Yohanes U. R.; Serangmo, Diana Y. L.
JURNAL AGRISA Vol 13 No 1 (2024): Jurnal Agrisa
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/agrisa.v13i1.10918

Abstract

Tomato plays an important role in Indonesian agriculture. Tomato production has been constrained by diseases, one of which was bacterial wilt (Ralstonia solanacearum). Application of Vesicular Arbuscular Mycorhiza (VAM) has been reported to be effective in suppressing diseases and can improve plant growth. This study was aimed to test the effect of VAM Glomus sp. in suppressing bacterial wilt disease on tomato (Lycopersicum esculentum). This research was conducted at Plant Disease Laboratory and Screenhouse of Agriculture Faculty, Nusa Cendana University. Seven treatments with three replications were arranged in Randomized Completely Block Design experiment. The treatments were 5 grams, 10 grams, and 15 grams of Glomus sp. per polybag that applied 1-week before pathogen inoculation, and also 5 grams, 10 grams, and 15 grams of Glomus sp. per polybag that applied simultaneously with pathogen inoculation. Control treatment was only inoculated with the pathogen. Three polybags, with two tomato plants planted in each were considered as one experimental unit. Ralstonia solanacearum was isolated from diseased tomato plant. Isolate pathogenicity was confirmed through hypersensitivity test on tobacco plants and on 21 day-old tomato plants. Two-week old tomato plants were inoculated with 15mL of suspension. The stem was punctured with sterile needle pryor inoculation. The result showed that Glomus sp. application before pathogen icoculation lowered disease incidence significantly. Application of 15 grams Glomus sp. before pathogen inoculation caused the greatest mycorrhizal vesicle formation in tomato root, which might also increased plant height and the number of leaves significantly.
Co-Authors Iburuni, Yohanes U. R. Kolo, Maria Serliana Serangmo, Diana Y. L. Wolagole, Sane