Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal Berkala Ilmiah Kimia Farmasi
Amirudin Prawita
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Chemistry Medicine & Pharmacology

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Determination of Queercetin Content in Preparation of Powder Extract of Starfruit Leaves (Averrhoa bilimbi L.) by Tlc-Densitometry Sudjarwo, Sudjarwo; Zulfiyah Emaz; Amirudin Prawita
Berkala Ilmiah Kimia Farmasi Vol. 10 No. 1 (2023): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikfar.v10i1.51432

Abstract

Quercetin was a flavonoid from Averrhoa bilimbi Linn. which had a variety of potential therapeutic uses. Therefore, this research was needed to determine the quercetin content in Averrhoa bilimbi L.’s extract powder. Method validation was performed to ensure that an analytical method met the criteria and requirements of a good analysis method. Method validation used was the first category with parameters of selectivity, linearity, accuracy, and precision. The content of quercetin in Averrhoa bilimbi L.’s leaf was small, which required the parameters of Limit of Detection (LOD) and Limit of Quantitation (LOQ). The analysis method was by TLC-Densitometry with Silica gel 60 F254, eluent of toluene: ethyl acetate: formic acid (7:3:1) as the mobile phase with a resolution of 1,67 and 1,60, respectively. The results showed a linear regression of y = 8122,4899 x + 1181,9943 (r: 0,9976; Vxo 4,88%). The accuracy was 90,2% (w/w) ± 5,89; the coefficient of variation of precision was 1,35%; LOD and LOQ were 0,0064 µg, and 0,0215 µg, respectively. The determination result showed that quercetin content in the leaf extract in ethanol of Averrhoa bilimbi L. was 0,58% (w/w) ± 0,04, and the determination of quercetin from 4,0 g extract powder of Averrhoa bilimbi L. leaf showed that the average content of quercetin was 6,05 ± 0,09 mg. Keywords:  Quercetin, Extract Powder, Averrhoa bilimbi L, TLC-Densitometry
Co-Authors Sudjarwo Sudjarwo Zulfiyah Emaz