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All Journal Medicra (Journal of Medical Laboratory Science/Technology) Stigma : Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa
Zaki, Ekiq Naufal
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Health Professions Mathematics Public Health

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DETECTION OF HETEROTROPHIC AND COLIFORM BACTERIA IN TWO RIVERS IN SIDOARJO REGENCY, INDONESIA: BUNTUNG AND SINIR RIVERS YACHYA, ARIF; Zaki, Ekiq Naufal; Herdina, Abelya Putri; Astuti, Yola Amanda; Azmi, Nabila Afni Ulul; Wakhyuni, Indah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 16 No 2 (2023)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.16.2.8609.73-79

Abstract

The Buntung and Sinir Rivers are located in Sidoarjo Regency, East Java. Until now, both rivers have been used by residents for their daily activities. Therefore, for the safety and health of residents living around these two rivers, it is necessary to carry out HPC (heterotrophic plate count) and coliform tests. The method used is the pour plate and MPN (Most Probable Number) series 5-5-5 tubes. The HPC test results showed that the population of heterotrophic bacteria in the Buntung and Sinir River water samples was 9,5,103 and 7,2,104 CFU/mL, respectively. The MPN values for total coliforms from Buntung and Sinir river water samples were 33.103 and 109.103 CFU/100 mL, respectively. The MPN values for fecal coliforms and E. coli are the same. The MPN values of fecal coliform and E. coli for Buntung and Sinir river water samples were 17.103 and 12.103 CFU/100 mL, respectively. These results show that the Buntung and Sinir Rivers are polluted by organic materials and feces. The population of heterotrophic bacteria and the total MPN coliform, fecal, and E. coli values for both river waters exceed the microbiological standards of Minister of Health Regulation Number 2 of 2023 and Republic of Indonesia Government Regulation Number 22 of 2021, so it is not suitable for daily activities.
Capacity of Mangrove Fruit Macerate (Sonneratia alba) as a Preservative Fresh Vannamei Shrimp (Litopenaeus vannamei) Meat: Kapasitas Maserat Buah Mangrove (Sonneratia alba) sebagai Pengawet Daging Udang Vannamei (Litopenaeus vannamei) Segar Wicaksono, Agung; Pramudya, Arkan Setia; Zaki, Ekiq Naufal; yachya, Arif
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 6 No. 2 (2023): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v6i2.1725

Abstract

Mangrove fruit (Sonneratia alba) is known to contain tannins. This flavonoid has antibacterial properties. It makes this fruit macerate has potential as a preservative for shrimp meat. This study aimed for determining the ability of S. alba fruit macerate to maintain the quality of vannamei shrimp meat. The study was conducted in a laboratory by exposing fresh vannamei shrimp meat to S. alba fruit macerate. Exposure was carried out by soaking shrimp meat. The concentrations of macerate used were 25, 50, 75, and 100%. The duration of soaking was 60 and 120 minutes. Bacterial population (total plate count), presence of hydrogen sulfide gas (H2S), acidity value (pH), and score of organoleptic were used as indicators of Shrimp meat quality. The tests were conducted after 24 hours of incubating shrimp meat at room temperature. The results showed the concentration of macerate and the time of soaking affected the quality of the shrimp meat. These effects were in the form of inhibition of bacterial populations, production of H2S and maintaining organoleptic properties, while the pH values ​​of all treatments were the same. Finally, the best concentration of macerate to maintain the quality of fresh vannamei shrimp meat is 100% with 60 minutes of soaking time.
Co-Authors Agung Wicaksono Arif Yachya Astuti, Yola Amanda Azmi, Nabila Afni Ulul Herdina, Abelya Putri Pramudya, Arkan Setia Wakhyuni, Indah