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Diah Titik , Mutiarawati
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology

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ANALISA BAKTERI Escherichia coli DAN Salmonella sp PADA TELUR AYAM KAMPUNG Damayanti, Della Ika Putri; Diah Titik , Mutiarawati; Suliati, Suliati
Analis Kesehatan Sains Vol. 10 No. 1 (2021): Analis Kesehatan Sains
Publisher : Politeknik Kesehatan Kementerian Kesehatan Surabaya

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Abstract

Meanings of animal origin are often contaminate by microorganism, one of it is chicken eggs. Free range chicken is often consumed raw for medicinal purposes without knowing any contamination in it. Free range chicken are often associated with the genesis of foodborne diseases that can cause ailments in the form of diarrheal and gastroenteristic diseases. According to data provided by the 2018 ministry of health, there are approximately 7 million cases of diarrheal disease every year. The bacteria responsible for diarrhea is Salmonella sp, Escherichia coli and Staphylococcus aureus. This type of research is laboratory experimental research through culture. The population of this study was eggs obtained from the village of Tanggul Kulon RT 01/RW 07 Jember. The sample of this research is free range chicken taken by purposive sampling based on criteria. The place of this research was conducted at the Bacteriology Laboratory of Poltekkes Surabaya from January to April 2021. The result showed 4 out of 12 positive samples identified as Escherichia coli and 8 out of 12 positive samples identified as Salmonella parathypi A bacteria
UJI OPTIMALISASI DAYA HAMBAT EKSTRAK DAUN TEH HIJAU (Camellia Sinensis) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli DAN Staphylococcus aureus HD. Widya , Salsyabillah Ramadhani; Diah Titik , Mutiarawati; Suliati, Suliati
Analis Kesehatan Sains Vol. 10 No. 2 (2021): Analis Kesehatan Sains
Publisher : Politeknik Kesehatan Kementerian Kesehatan Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/anakes.v10i2.17

Abstract

Research on substances as new antibiotics to inhibit or kill bacteria that are resistant to antibiotics needs to be done. One of the simplicia has an antibacterial effect is the leaves of green tea (Camellia sinensis). Antibacterial content the leaves of green tea (Camellia sinensis), namely alkaloids, saponins, tannins, and catechins (polyphenols). Tea one of the favorite products from Indonesia’ estate. The purpose of this study was to determine optimization of the inhibitory power the leaves of green tea’s (Camellia sinensis) extract against the growth of Escherichia coli and Staphylococcus aureus. This research is an experimental laboratory conducted at Bacteriology”s Laboratory, Department of Medical Laboratory Technology Surabaya in November 2020 - April 2021. This study uses the Kirby Bauer disk diffusion method, namely Mueller Hinton Agar (MHA) media which has been planted with Escherichia coli and Staphylococcus aureus with disks that have been prepared. soaked in the leaves of green tea’s (Camellia sinensis) extract at concentrations of 25%, 50%, 75%, and 100% and then incubated for 24 hours in an incubator. The results can be concluded the leaves of green tea’s (Camellia sinensis) extract can be used as an antibacterial. Optimal of results in this research were Escherichia coli at a concentration of 25% with an average diameter of 10,25 mm and Staphylococcus aureus at a concentration of 100% with an average of 22,5 mm. This is because content the leaves of green tea’s (Camellia sinensis) is catechins and alkaloids as strong antibacterials
Co-Authors Damayanti, Della Ika Putri HD. Widya , Salsyabillah Ramadhani Suliati Suliati