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Anita Dwi , Anggraini
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology

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POTENSI METABOLIT SEKUNDER ANTIFUNGI AKTINOMISETES YANG DIISOLASI DARI TANAH MANGROVE WONOREJO SURABAYA TERHADAP Trichophyton rubrum Fitria, Febrianti; Retno, Sasongkowati; Anita Dwi , Anggraini
Analis Kesehatan Sains Vol. 10 No. 1 (2021): Analis Kesehatan Sains
Publisher : Politeknik Kesehatan Kementerian Kesehatan Surabaya

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Abstract

Actinomycetes are gram-positive bacteria that have aerobic properties. Actinomycetes have the ability to produce antibiotic compounds. Actinomycetes are the second largest organisms that live in soil with a population of 1-10 million per gram of soil. Actinomycetes also make up 10-50% of the microbes in the soil. The purpose of this study was to determine the antifungal activity of actinomycetes isolates isolated from the soil of the Mangrove Wonorejo forest in Surabaya against the fungus Trichophyton rubrum. This type of research is an experimental laboratory with quantitative analysis. Actinomycetes isolates were taken from 3 different soil locations, pretreatment by heat treatment at 90°C for 15 minutes. Actinomycetes were isolated on casein starch agar medium which had been added with 0.002% Nystatin. Selection of antimicrobial compounds was based on the disc diffusion method which was characterized by the formation of a zone of inhibition around the disc. The results showed that from 3 locations of soil sampling, 8 actinomycetes isolates were obtained, but only 6 isolates were able to inhibit the fungus Trichophyton rubrum. showed that isolates C3-2, B3-1, C4-1, were categorized as having very strong inhibitory power. While isolates A5-1, A3-2 were categorized as having inhibitory power and isolates A4-1 and A4-2 were categorized as having moderate inhibitory power against the fungus Trichophyton rubrum.
HUBUNGAN KEJADIAN INFEKSI Soil transmitted helminths (STH) dengan NILAI EOSINOFIL dan HEMOGLOBIN DARAH pada MASYARAKAT di KAMPUNG 1001 MALAM SURABAYA Arin, Okvitasari; Retno, Sasongkowati; Anita Dwi , Anggraini
Analis Kesehatan Sains Vol. 10 No. 2 (2021): Analis Kesehatan Sains
Publisher : Politeknik Kesehatan Kementerian Kesehatan Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/anakes.v10i2.19

Abstract

Soil worm infection transmitted Helminths (STH) is a group of parasitic nematodes that cause infection in the human gut which is transmitted through contaminated soil eggs or larvae. STH often infects people with poor environmental sanitation and lack of public awareness of the importance of maintaining personal and environmental hygiene. An increase in the value of eosinophils and a decrease in the value of blood hemoglobin are markers of infection by the STH parasite. The purpose of this study was to determine the relationship between the incidence of soil-transmitted helminths (STH) infection with the value of eosinophils and blood hemoglobin in the 1001 night village community, Surabaya. This type of research is an analytical observational study and the samples in this study were blood and feces taken from the people of 1001 Malam Surabaya village as many as 25 people with a random sampling technique using the native method for stool examination and using a hematology analyzer for blood examination. Based on the results of research conducted on the 1001 night village community, it was known that the prevalence of worm infection was Ascaris lumbricoides. The prevalence of STH infection with eosinophilia was 15 samples. A significant relationship after being tested statistically with the Spearman Rank Correlation test was found between STH infection and the eosinophil value of 0.034. Then on the relationship between hemoglobin values and STH infection, a significant value was obtained, namely 0.00. There is a significant relationship between STH infection with the value of eosinophils and blood hemoglobin.
Co-Authors Arin, Okvitasari Fitria, Febrianti Retno, Sasongkowati