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Monotilla, Alvin

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Author-ID : 7405783

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Veterinary

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New Report of Endophytic Sordariomycetes from the Seagrasses of Cebu, Central Philippines: Endophytic Sordariomycetes from Philippine seagrasses Kinamot, Venus Bantoto; Monotilla, Alvin
Journal of Tropical Life Science Vol. 13 No. 3 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.03.14

Sordariomycete is one of the dominant components of the endophytic community in seagrasses. However, there are still many species under this group that still need to be described. Among the tropical seagrasses, the Philippines had the least number of endophytic Sordariomycete reported. This study reports the isolation of endophytic Sordariomycete from Enhalus acoroides, Cymodocea serrulata, and Thalassia hemprichii. Seagrass samples were collected from the coastal areas of Hilutungan Channel, Cebu, Central Philippines using a sterilized razor. Explants from seagrass samples were surface-sterilized, inoculated in culture plates, and incubated for 14 days at 27±2°C. Fungi that grew from the edge of explants were purified and identified through morphological and molecular methods using ITS sequences. Molecular phylogeny was inferred by neighbor-joining with 1000 bootstrap replications. Three species of Sordariomycete were identified in this study. Fungal isolates from the rhizomes and roots of Cymodocea serrulata had 99.84% nucleotide similarity with Eutypella sp. Isolates from the rhizomes and roots of Enhalus acoroides had a 100% match identity with Beauveria bassiana. While the isolate from the leaf of Thalassia hemprichii had a 99% nucleotide affinity to Xylaria apoda. These isolates formed a well-supported clade with their closest match taxa at 100% bootstrap support. The estimate of evolutionary divergence between the isolates’ sequences and their reference taxa was 0.0, suggesting no nucleotide base substitutions per site. The identification of seagrass isolates as Eutypella sp., Beauveria bassiana, and Xylaria apoda is hereby supported. Phylogenetic analysis revealed that endophytic fungi from the Philippine seagrass did not cluster with any fungal species previously reported from the Philippines, suggesting different species. To our knowledge, Eutypella sp., Beauveria bassiana, and Xylaria apoda were the first reports of endophytic Sordariomycete in Philippine seagrasses. The isolation of these endophytes is promising because these genera are a rich source of novel compounds and biocontrol agents.

New Report of Endophytic Sordariomycetes from the Seagrasses of Cebu, Central Philippines: Endophytic Sordariomycetes from Philippine seagrasses Kinamot, Venus Bantoto; Monotilla, Alvin
Journal of Tropical Life Science Vol. 13 No. 3 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.03.14

Sordariomycete is one of the dominant components of the endophytic community in seagrasses. However, there are still many species under this group that still need to be described. Among the tropical seagrasses, the Philippines had the least number of endophytic Sordariomycete reported. This study reports the isolation of endophytic Sordariomycete from Enhalus acoroides, Cymodocea serrulata, and Thalassia hemprichii. Seagrass samples were collected from the coastal areas of Hilutungan Channel, Cebu, Central Philippines using a sterilized razor. Explants from seagrass samples were surface-sterilized, inoculated in culture plates, and incubated for 14 days at 27±2°C. Fungi that grew from the edge of explants were purified and identified through morphological and molecular methods using ITS sequences. Molecular phylogeny was inferred by neighbor-joining with 1000 bootstrap replications. Three species of Sordariomycete were identified in this study. Fungal isolates from the rhizomes and roots of Cymodocea serrulata had 99.84% nucleotide similarity with Eutypella sp. Isolates from the rhizomes and roots of Enhalus acoroides had a 100% match identity with Beauveria bassiana. While the isolate from the leaf of Thalassia hemprichii had a 99% nucleotide affinity to Xylaria apoda. These isolates formed a well-supported clade with their closest match taxa at 100% bootstrap support. The estimate of evolutionary divergence between the isolates’ sequences and their reference taxa was 0.0, suggesting no nucleotide base substitutions per site. The identification of seagrass isolates as Eutypella sp., Beauveria bassiana, and Xylaria apoda is hereby supported. Phylogenetic analysis revealed that endophytic fungi from the Philippine seagrass did not cluster with any fungal species previously reported from the Philippines, suggesting different species. To our knowledge, Eutypella sp., Beauveria bassiana, and Xylaria apoda were the first reports of endophytic Sordariomycete in Philippine seagrasses. The isolation of these endophytes is promising because these genera are a rich source of novel compounds and biocontrol agents.

IDENTIFICATION AND DIVERSITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH THE SEAGRASSES OF CEBU, CENTRAL PHILIPPINES Kinamot, Venus; Monotilla, Alvin
BIOTROPIA Vol. 30 No. 1 (2023): BIOTROPIA Vol. 30 No. 1 April 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.1.1861

Endophytic fungi were poorly documented in the marine environment, especially in seagrasses regardless of their importance as sources of novel metabolites. In the Philippines, studies are dearth despite having large areas of seagrass meadows. Thus, this study was conducted to isolate and identify endophytic fungi associated with common seagrasses: Enhalus acoroides, Cymodocea serrulata, and Thalassia hemprichii from the Philippines by morphologic and molecular methods. Seven species were identified in this study: Aspergillus tamarii, A. ochraceopetaliformis, A. sydowii, Penicillium citrinum, Xylaria sp., Beauveria bassiana, and Eutypella sp. Morphologically, Aspergillus spp. had septate hyphae, biseriate conidiophore, and smooth to rough globose conidia. Penicillium had a green colony, biverticillate conidiophore, and smooth, globose conidia. Beauveria bassiana had white to cream colonies with irregular edges and a powdery appearance. The conidiogenous cells had zigzag rachis in which a chain of conidia emerged. Eutypella sp. had white, filariform, and plumose colony. Xylaria sp. had a white colony with conspicuous radial lines and a plumose margin. Phylogenetic analysis using 80 ITS rDNA sequences by neighbor-joining revealed the clustering of our isolates with the closest match taxa in the same clade with 100% bootstrap value. The estimate of evolutionary divergence between our isolates and their closest match taxa by pairwise distance showed no nucleotide base substitution suggesting high sequence identity between sequences. The most diverse endophyte is Aspergillus as it is ubiquitously adapted to the marine environment. To our knowledge, this is the first report of these species colonizing the seagrasses from the Philippines.

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