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All Journal Natural Science: Journal of Science and Technology Biocelebes KOVALEN: Jurnal Riset Kimia
Meryany Ananda
Jurusan Biologi Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Tadulako Kampus Bumi Tadulako Tondo Palu, Sulawesi Tengah
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT

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CHARACTERIZATION OF α-AMYLASE ACTIVITY FROM THERMOPHILIC BACTERIA ISOLATED FROM BORA HOT SPRING, CENTRAL SULAWESI Gazali, Faris Muhammad; Ananda, Meryany; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 7, No 1 (2018): Volume 7 Number 1 (March 2018)
Publisher : Univ. Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (610.516 KB)

Abstract

?-Amilase (EC 3.2.1.1) merupakan salah satu jenis enzim yang sangat penting dalam bioteknologi. Enzim ini sering diaplikasikan pada berbagai sektor industri, seperti industri deterjen, tekstil, kertas, roti, alkohol, dan pati. Termostabilitas dari ?-Amilase merupakan sifat utama dari enzim yang dibutuhkan pada industri pemerosesan pati. ?-Amilase dengan sifat termostabil dapat diperoleh dari bakteri termofilik yang secara alami dapat diisolasi dari mata air panas. Mata air panas Bora merupakan salah satu habitat yang paling berpotensi dalam mengisolasi bakteri termofilik karena memiliki suhu air yang sangat tinggi hingga mencapai 90,1°C. Penelitian ini bertujuan untuk mengetahui karakter aktivitas ?-Amilase dari bakteri termofilik lokal isolat mata air Bora, Sulawesi Tengah. Terdapat 13 (dari 18 isolat) bakteri termofilik isolat mata air panas Bora yang mampu menghasilkan ?-Amilase yang berhasil diisolasi dari sampel sedimen. Dari 13 isolat tersebut, 3 isolat terpilih dideskripsikan pada tulisan ini, yakni BR 001, BR 006, and BR 012. Ketiga isolat tersebut memiliki karakter: nilai indeks amilolitik berkisar 0,78 ? 1,44; aktivitas ?-Amilase berkisar berkisar 3,67 U/ml ? 7,6 U/ml; konsentrasi protein berkisar 0,02 mg/ml ? 0,14 mg/ml; aktivitas spesifik enzim kasar ?-Amilase berkisar 54,83 U/mg ? 308,45 U/mg; ?-Amilase yang aktif pada kisaran suhu 32°C ? 80°C; dan ?-Amilase yang aktif pada kisaran pH 5 ? 7 dan memiliki pH optimum pada kisaran 6,5 ? 7. Adanya aktivitas enzim pada suhu yang tinggi dari ?-Amilase yang diproduksi oleh isolat bakteri termofilik lokal dari mata air panas Bora menunjukkan adanya potensi dalam aplikasi enzim lokal pada sektor industri di masa depan, khususnya pada industri pemerosesan pati.
Fermentasi Kelapa Parut Bebas Protein dengan Aspergillus niger untuk Menghasilkan Lipase: Fermentation of Protein-Free Grated Coconut with Aspergillus niger to Produce of Lipase Rifka Aulia; Syaiful Bahri; Meryany Ananda
KOVALEN: Jurnal Riset Kimia Vol. 6 No. 1 (2020): Edisi April
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2020.v6.i1.13052

Abstract

The research about the fermentation of protein-free grated coconut with Aspergillus niger to produce of crude lipase enzyme has been carried out. This study aims to determine the effect of water content on fermentation medium and the incubation time which results lipase enzyme extract with the highest activity. This study used a Completely Randomized Design (CRD) consisting of 2 factors, i.e. variations of water content in medium of 40%, 50%, 60%, and 70% and incubation times of 48, 96, 144, and 192 hours. The parameters observed were lipase enzyme activity produced in each treatment. The results showed that the highest medium water content was 70% with the lipase enzyme activity 4.33 µmol/mL.minute and the best incubation time was 96 hours with the lipase enzyme activity of 0.83 µmol/mL.minute. Keywords: Lipase enzyme, protein-free grated coconut, Aspergillus niger
PENGAMATAN PERTUMBUH MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus (Jacq). P.kummer) PADA MEDIA DASAR SERBUK GERGAJI DENGAN SUPLEMENTASI AMPAS SAGU Wahyu Hidayat; Umrah Umrah; Meryany Ananda
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.415 KB) | DOI: 10.22487/bioceb.v13i3.14966

Abstract

The study of observation of growth media formulation of white oyster mushrooms’s mycelium (Pleurotus ostreatus (Jacq.) P.Kummer) on the base with sago pulp supplementation medium was conducted from March to May 2018 at Biotechnology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. This study were aimed to determine the appropriate formulation and dosage as supplementation of sago pulp with basic media for the growth of the white oyster mushroom mycelium. This study was conducted by Randomized Complete Random Design (RAL), consisted of seven treatments and four replications with basic media and supplementary M0 (100%: without supplements), M1 (95% : 5%), M2 (90% : 10%), M3 (85% : 15%), M4 (80% : 20%), M5 (75% : 25%), M6 (70% : 30%). The results showed that the best growth of mycelium on treatment M0 and M1, mycelium filled the media for 26 days with the average growth of mycelium M0 and M1 were 0,56 cm/ day. The best formulation and dosage of sago suplementation on the base medium were found in M1 (95% base medium: 5% sago pulp).
POTENSI EKONOMI PEMANFAATAN LIMBAH BATANG PISANG DAN LIMBAH JERAMI JAGUNG SEBAGAI SUBSTRAT DASAR UNTUK PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH umrah umrah; I Nengah Suwastika; Lina Mahardiana; Meryany Ananda; Novika
Biocelebes Vol. 15 No. 1 (2021)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v15i1.15490

Abstract

Pertumbuhan miselium jamur tiram putih (Pleurotus ostreatus) pada substrat (batang pisang dan batang jagung) telah dilakukan di Laboratorium Mikrobiologi, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam. Penelitian ini bertujuan untuk mengetahui: (a) pertumbuhan P. ostreatus pada substrat yang ditambahkan dengan limbah air kelapa dan limbah tempe; (b) karakteristik pertumbuhan P. ostreatus pada perlakuan berbagai jenis substrat. Penelitian ini dirancang berdasarkan Rancangan Acak Lengkap (RAL) yang terdiri dari enam perlakuan dan empat ulangan. Komposisi dari perlakuan adalah rasio antara batang pisang : batang jagung yaitu P1 (100%), P2 (80%: 20%), P3 (60%: 40%), P4 (40%: 60%), P5 (20% : 80%), P6 (100%). Hasil penelitian menunjukkan bahwa Perlakuan P6 lebih baik dibandingkan dengan perlakuan lain pada pertumbuhan miselium.
POTENSI EKONOMI LIMBAH TANDAN KOSONG KELAPA SAWIT DAN DEDAK PADI SEBAGAI MEDIA TUMBUH MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) umrah umrah; Lina Mahardiana; Meryany Ananda; Eny Yuniati; Mutma Inna
Biocelebes Vol. 15 No. 2 (2021)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v15i2.15607

Abstract

Penelitian ini perlu di lakukan karena pada limbah tandan kosong kelapa sawit dan dedak padi merupakan sumber nutrisi yang bernilai ekonomi tinggi yang dapat dimanfaatkan sebagai media pertumbuhan jamur tiram putih (Pleurotus ostreatus). Tujuan penelitian ini adalah (1) mengetahui pertumbuhan miselium jamur tiram putih (P. ostreatus) pada substrat limbah tandan kosong kelapa sawit dan dedak padi; (2) mengetahui karakteristik pertumbuhan jamur tiram putih (P. ostreatus) pada perlakuan jenis substrat yang berbeda. Penelitian ini telah di lakukan dalam rancangan acak lengkap (RAL) yang terdiri tujuh perlakuan dan tiga kali pengulangan. Susunan perlakuan merupakan perbandingan antara tandan kosong kelapa sawit : dedak padi, yakni P1 (50% : 50%), P2 (45% : 55%), P3 (40% : 60%), P4 (35% : 65%). P5 (30% : 70%), P6 (25% : 75%), P7 (serbuk geragaji 70%, dedak padi 20%, tepung jagung 10%). Adapun hasil penelitian menunjukkan laju pertumbuhan miselium tercepat pada perlakuan P1 yaitu 89 mm dan terendah pada perlakuan P5 yaitu 80 mm. Jumlah koloni tertinggi pada perlakuan P5 yaitu 1,4x1012 CFU/g dan terendah pada perlakuan P4 yaitu 0,4x1012 CFU/g. Waktu inkubasi paling cepat pada perlakuan P4 yaitu 28 hari dan terendah pada perlakuan P7 yaitu 33 hari.
PENGARUH PUPUK HAYATI DENGAN KOMPOSISI MIKROORGANISME YANG BERBEDA TERHADAP PERTUMBUHAN TANAMAN Amalia Puji` Cahyani; Asri Pirade Paserang; Meryany Ananda; Wahyu Harso
Biocelebes Vol. 15 No. 2 (2021)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v15i2.15781

Abstract

Biofertilizer can be used to replace chemical fertilizer so that soil quality is maintained and soil pollution can be avoided. The study aimed to determine the effect of biofertilizers with different compositions of microorganisms on the growth of maize plants. The research was carried out in a greenhouse. Maize plants were fertilized by two kinds of biofertilizers with different compositions of microorganisms. The first biofertilizer contained Azotobacter sp, Azospirillum sp, Rhizobium sp, Trichoderma sp, and Lactobacillus sp, and the second biofertilizer contained Strenotrophomonas sp. and Paenibacillus polymyxa. As controls, maize plants were fertilized by sterilized those biofertilizers. The results showed that the second biofertilizer increased the dry weight of the maize plant. Meanwhile, the first biofertilizer did not increase it. Several things must be considered in the application of biofertilizers, such as the composition of microorganisms, type of plant, level of fertilization, and the method of applying fertilizer to the plant.
MEDIA DASAR LIMBAH DAUN KAKAO UNTUK PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) Umrah; Saparudin, Saparudin; Yuniati, Eny; Ananda, Meryany
Biocelebes Vol. 17 No. 2 (2023)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v17i2.16606

Abstract

Cocoa leaf wastes as a basic medium are the nutrition that needed for the growth of white oyster mushroom mycelium (Pleurotus ostreatus). The objectives of this study were determine the cocoa leaf wastes as a basic medium for the growth of white oyster mushroom mycelium and the dosage of basic medium to encourage the best fungal growth. This research was designed in a Completely Randomized Design (CRD), consisting of seven treatments and three replications, namely P1 (Basic Media of 100% cocoa leaf waste), P2 (Basic Media 90% + Suplement 10%), P3 (Basic Media 80% + Suplement 20 %), P4 (Basic Media 70% + Suplement 30%), P5 (Basic Media 60% + Suplement 40%), P6 (Basic Media 50% + Suplement 50%), P7 (Sawdust 70%, 20% rice bran + flour corn 10%). The method that used in this study were media preparation, drying, grinding and formulation. The parameters that observed were the basic media, microscopic characteristics of hyphae, mycelium growth curve and macroscopic characteristics of colonies. The results of P1 and P7 showed the best growth for mycelium, observation of mycelium growth were done every 3 days. The isolation stage was carried out on Potato Sucrose Agar (PSA) media, the results obtained that P1 was 0,1 x 1012 CFU/g, P2 was 0,4 x 1012 CFU/g, P3 was 0,3 x 1012 CFU/g, P4 as much as 0,7 x 1012 CFU/g, P5 as much as 0,6 x 1012 CFU/g, P6 as much as 0,4 x 1012 CFU/g, P7 as much as 2,4 x 1012 CFU/g, respectively. Cocoa leaf waste are potentially as a growth medium for mycelium of white oyster mushroom.
APLIKASI KOMPOS CAIR AEROB DAN ANAEROB PADA PERTUMBUHAN TANAMAN JAGUNG: Application of Aerobic and Anaerobic Liquid Compos on Corn Plant Growth Wasilahturahman; Harso, Wahyu; Umrah; Ananda, Meryany
Biocelebes Vol. 17 No. 2 (2023)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v17i2.16611

Abstract

The price of chemical fertilizers and potential environmental risks are the reasons for using compost. Liquid compost extracted from compost has many advantages that a compost. Liquid compost is available quickly and has a higher concentration compared to compost. The effectiveness of liquid compost depends on the aeration treated in the production process. The study aimed to determine the growth of corn plants fed with either aerated liquid compost or an aerated liquid compost. This study was conducted by a Complete Randomized Design with two factors. The first factor is the type of liquid compost, aerobic and anaerobic liquid compost. The second factor is the dose of a liquid compost, 100 ml and 200 ml of liquid compost. The growth medium was drenched with aerobic or anaerobic liquid compost in the first and third weeks after planting. The results showed that plants fertilized by aerobic or anaerobic liquid compost increased plant growth. The plant fertilized by anaerobic liquid compost had higher plant growth than it fertilized by aerobic liquid compost. Key words: aerobic, anaerobic, corn plants, liquid compost Harga pupuk kimia dan potensi resiko lingkungan merupakan alasan penggunan kompos. Kompos cair merupakan ekstrak dari kompos memiliki keuntungan yang tidak dimiliki oleh kompos yaitu tersedia dengan cepat dan memiliki konsentrasi yang lebih tinggi dibandingkan dengan kompos. Efektifitas kompos cair tergantung dari aerasi yang diberikan pada proses pembuatannya. Tujuan dari penelitian ini adalah untuk mengetahui pertumbuhan tanaman jagung yang diberi kompos cair yang dibuat secara aerob dan anaerob. Penelitian dilakukan dengan menggunakan Rancangan Acal Lengkap dengan dua faktor. Faktor pertama adalah jenis kompos cair yaitu kompos cair aerob dan anaerob. Faktor kedua adalah dosis pemberian kompos cair yaitu 100 ml dan 200 ml. Media tanam tanaman jagung diberi kompos cair aerob atau anaerob di minggu pertama dan ketiga setelah tanam. Hasil penelitian menunjukkan bahwa pemberian kompos cair aerob atau anaerob meningkatkan pertumbuhan tanaman. Peningkatan pertumbuhan tanaman lebih tinggi terjadi pada tanaman yang diberi kompos cair anaerob dibandingkan dengan yang diberi kompos cair anaerob. Kata kunci : aerob, anaerob, pupuk cair, tanaman jagung
Co-Authors Amalia Puji` Cahyani Asri Pirade Paserang Eny Yuniati Gazali, Faris Muhammad I Nengah Suwastika Lina Mahardiana Mutma Inna Novika Rifka Aulia Saparudin Saparudin Syaiful Bahri Umrah Umrah Wahyu Harso Wahyu Hidayat Wasilahturahman