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All Journal JMM (Jurnal Masyarakat Mandiri) Eksakta : Berkala Ilmiah Bidang MIPA Medical and Health Journal Journal of Clinical Microbiology and Infectious Diseases
Meike Tiya Kusuma
Kebidanan, Universitas Negeri Surabaya
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Other

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Effect of oral administration of in silico epitope-based SARS-CoV-2 virus with ISCOM adjuvants on increasing the number of NK cells and serum IgG in mice Irma Nur Sukmawati; Meike Tiya Kusuma; Khoirul Anam; Sumarno Retro Prawiro
Journal of Clinical Microbiology and Infectious Diseases Vol. 3 No. 2 (2023): Available online: December 2023
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v3i2.34

Abstract

Background: Vaccines are one of the best solutions to deal with the COVID-19 pandemic. Epitope vaccines can be searched in silico. The selection of in silico epitope-based SARS-CoV-2 which is used as a vaccine candidate must be able to trigger an immune response, such as proteins from the spike (S), envelope (E), membrane (M) in SARS-CoV-2. This study aims to determine the potential for in silico epitope-based SARS-CoV-2 from S, EM, and SEM which is immunogenic, non-toxic, and non-allergenic. And evaluate the immune response by measuring the number of NK cells in the spleen and serum IgG levels in mice. Method: This research was carried out in 2 stages, an in silico exploratory study and an experimental study. The exploratory stage consisted of selecting immunogenic, non-toxic, non-allergic vaccine candidates, molecular docking tests, and epitope conjugation with an adjuvant in the form of ISCOM which was observed with a TEM microscope. The first group was the control, and the second group was given ISCOM. The remaining groups were each given the S, EM, and SEM epitope which had been conjugated with ISCOM and all were given orally. In 5 groups, NK cell levels were measured using a flow cytometer, while IgG levels were measured using Elisa. Research: The results of the in-silico test showed that 3 epitopes of S (FLVLLPLVSSQCVN), E (VNSVLLFLAFVVFLLVTLASS), and M (LYIIKLIFLWLLWPVTLACFV-LAAVY) were immunogenic, non-toxic, and non-allergic. Oral administration of in silico epitope-based SARS-CoV-2 in mice could increase the highest number of NK cells in the administration of S epitope. Meanwhile, the highest serum IgG level was given with the combination of SEM epitope. Conclusion: Oral administration of an in-silico epitope based on SARS-CoV-2 from spike, envelope, and membrane can increase the number of NK cells in the spleen and IgG levels in mice.
Study of Indonesian Rhododendron: Classification, Conservation, and Pharmacology Activity Birhi, Damiana Nofita; Meike Tiya Kusuma; Antonia Fransiska Laka
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 26 No. 02 (2025): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol26-iss02/583

Abstract

Rhododendrons are representatives of ornamental plants with a wide range of pharmacological activities. Indonesia is the second richest country in Rhododendron plants with 233 species. Unfortunately, more than 85 species are severely lacking in data, 21 species are vulnerable, and more than 30 others are endangered or even no longer found. The purpose of this study is to review the species that have been found in Indonesia, and find the factors that affect conservation efforts to prevent the extinction of this plant. The results of the study succeeded in recording 221 species that had been found in Indonesia with 4 of them not having sufficient data so that named Rhododendron sp1, R. sp2, R. sp3 and R. sp4 by local residents. Four species were confirmed to be extinct, and most of the ex-situ conservation efforts unsuccessful. The results of this study show that there is need for cooperation between the government and residents around the Rhododendron growing location in an effort to preserve this plant. Researchers are also expected to pay more attention to this plant considering it has bioactive compounds with very high pharmacological properties.
In Silico Analysis of Moringa oleifera Leaf Phytochemicals as Potential DNA Gyrase Inhibitor in Salmonella typhi Kusuma, Meike Tiya; Umam, Muzid Syauqil; Birhi, Damiana Nofita
Medical and Health Journal Vol 5 No 2 (2026): February
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mhj.2026.5.2.19522

Abstract

Salmonella typhi is an infectious bacterium leading to typhoid fever, that is increasing in cases worldwide each year. Due to the resistance to antibiotics, the discovery of safer and efficacious drugs remains important. This study evaluates the potential interaction of M. oleifera phytochemical compounds against DNA gyrase of Salmonella typhi using an in silico molecular docking approach. Ligand’s phytochemicals were retrieved from the PubChem database, while the three-dimensional structure of DNA Gyrase subunit A and subunit B were obtained from the Protein Data Bank (PDB ID: 5ZJT and 6J90). Molecular docking was done using PyRx software and BIOVIA Discovery Studio as visualization software. Phytochemical compounds that had higher predictive binding affinity to DNA gyrase subunit A were Naringenin (∆G=-7.9 kcal/mol), 6-Prenylnaringenin (∆G=-8.1 kcal/mol), 6-Methoxypodophyllotoxin (∆G=-7.9 kcal/mol). Brefeldin A- DNA gyrase subunit A complex had the same binding affinity to ciprofloxacin (∆G=-7.7 kcal/mol). Phytochemical compounds that had higher predictive binding affinity to DNA gyrase subunit B were Naringenin (∆G=-8.9 kcal/mol), 6-Prenylnaringenin (∆G=-8.8 kcal/mol), 3',4'-Dimethoxy-7-hydroxyflavone (∆G=-8.6 kcal/mol), Sinapoyl malate (∆G=-7.8 kcal/mol), and Sinapoyl malate-4'-methyl ester (∆G=-8.3 kcal/mol). These findings suggest that phytochemical compounds from M. oleifera leaves may act as potential DNA gyrase inhibitors. However, further in vitro and in vivo validation is required to confirm its antibacterial activity.
SKRINING KESEHATAN DIGITAL: ALAT BARU PENCEGAHAN FEMALE ATHLETE TRIAD SYNDROME DALAM MEWUJUDKAN KESEHATAN HOLISTIK ATLET WANITA Syamsudi, Nur Anindya; Wahyuni, Sri; Chuvita, Laila; Kusuma, Meike Tiya; Sholikhah, Desty Muzarofatus; Antoni, Muhamad Fauzi; Wijaya, Fransisca Januarumi Marhaendra; Syamsudi, Faizatuz Azzahrah
JMM (Jurnal Masyarakat Mandiri) Vol 10, No 2 (2026): April
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v10i2.38487

Abstract

Abstrak: Female Athlete Triad (FAT) Syndrome merupakan masalah kesehatan kritis pada atlet wanita dengan prevalensi 40-60%, namun kesadaran dan skrining dini masih terbatas. Pengabdian ini bertujuan mengimplementasikan skrining kesehatan digital untuk pencegahan FAT Syndrome dalam mewujudkan kesehatan holistik atlet wanita. Kegiatan dilaksanakan secara hybrid melalui online class dan offline class pendampingan penggunaan skrining digital pada 15 atlet wanita. Evaluasi dilakukan melalui angket respon terhadap fasilitator, skrining digital, dan metode kegiatan. Hasil menunjukkan seluruh peserta (100%) menilai skrining digital mudah digunakan dan puas terhadap pendampingan fasilitator. Metode offline class (98%) lebih menarik dibanding online class (89,8%). Namun, 67,3% peserta menilai isi skrining digital kurang lengkap, terutama fitur nutrisi dan fitur kesehatan tulang. Pengembangan ke depan perlu menambahkan ukuran rumah tangga (URT) dan gambar porsimetri pada fitur nutrisi, serta meringkas pertanyaan fitur kesehatan tulang. Studi dengan periode observasi lebih panjang dan sampel lebih besar diperlukan untuk mengevaluasi efektivitas jangka panjang.Abstract: Female Athlete Triad (FAT) Syndrome is a critical health issue among female athletes, with a prevalence of 40–60%; however, awareness and early screening remain limited. This community service initiative aims to implement digital health screening for the prevention of FAT Syndrome to promote holistic health among female athletes. The activity was conducted in a hybrid format through online classes and offline classes providing guidance on the use of digital screening for 15 female athletes. Evaluation was conducted via a questionnaire assessing responses to the facilitator, the digital screening, and the activity methods. The results showed that all participants (100%) rated the digital screening as easy to use and were satisfied with the facilitator’s guidance. The offline class method (98%) was found to be more engaging than the online class (89.8%). However, 67.3% of participants rated the content of the digital screening as incomplete, particularly the nutrition and bone health features. Future development should include household portion sizes (HPS) and portion-measuring images in the nutrition feature, as well as streamline the questions in the bone health feature. Studies with longer observation periods and larger samples are needed to evaluate long-term effectiveness.
Co-Authors Aminuyati Antoni, Muhamad Fauzi Antonia Fransiska Laka Chuvita, Laila Damiana Nofita Birhi Irma Nur Sukmawati Sholikhah, Desty Muzarofatus Sri Wahyuni Sumarno Retro Prawiro Syamsudi, Faizatuz Azzahrah Syamsudi, Nur Anindya Umam, Muzid Syauqil Wijaya, Fransisca Januarumi Marhaendra