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All Journal Eksakta : Berkala Ilmiah Bidang MIPA Journal of Clinical Microbiology and Infectious Diseases
Meike Tiya Kusuma
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Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering

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Effect of oral administration of in silico epitope-based SARS-CoV-2 virus with ISCOM adjuvants on increasing the number of NK cells and serum IgG in mice Irma Nur Sukmawati; Meike Tiya Kusuma; Khoirul Anam; Sumarno Retro Prawiro
Journal of Clinical Microbiology and Infectious Diseases Vol. 3 No. 2 (2023): Available online: December 2023
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v3i2.34

Abstract

Background: Vaccines are one of the best solutions to deal with the COVID-19 pandemic. Epitope vaccines can be searched in silico. The selection of in silico epitope-based SARS-CoV-2 which is used as a vaccine candidate must be able to trigger an immune response, such as proteins from the spike (S), envelope (E), membrane (M) in SARS-CoV-2. This study aims to determine the potential for in silico epitope-based SARS-CoV-2 from S, EM, and SEM which is immunogenic, non-toxic, and non-allergenic. And evaluate the immune response by measuring the number of NK cells in the spleen and serum IgG levels in mice. Method: This research was carried out in 2 stages, an in silico exploratory study and an experimental study. The exploratory stage consisted of selecting immunogenic, non-toxic, non-allergic vaccine candidates, molecular docking tests, and epitope conjugation with an adjuvant in the form of ISCOM which was observed with a TEM microscope. The first group was the control, and the second group was given ISCOM. The remaining groups were each given the S, EM, and SEM epitope which had been conjugated with ISCOM and all were given orally. In 5 groups, NK cell levels were measured using a flow cytometer, while IgG levels were measured using Elisa. Research: The results of the in-silico test showed that 3 epitopes of S (FLVLLPLVSSQCVN), E (VNSVLLFLAFVVFLLVTLASS), and M (LYIIKLIFLWLLWPVTLACFV-LAAVY) were immunogenic, non-toxic, and non-allergic. Oral administration of in silico epitope-based SARS-CoV-2 in mice could increase the highest number of NK cells in the administration of S epitope. Meanwhile, the highest serum IgG level was given with the combination of SEM epitope. Conclusion: Oral administration of an in-silico epitope based on SARS-CoV-2 from spike, envelope, and membrane can increase the number of NK cells in the spleen and IgG levels in mice.
Study of Indonesian Rhododendron: Classification, Conservation, and Pharmacology Activity Birhi, Damiana Nofita; Meike Tiya Kusuma; Antonia Fransiska Laka
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 26 No. 02 (2025): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol26-iss02/583

Abstract

Rhododendrons are representatives of ornamental plants with a wide range of pharmacological activities. Indonesia is the second richest country in Rhododendron plants with 233 species. Unfortunately, more than 85 species are severely lacking in data, 21 species are vulnerable, and more than 30 others are endangered or even no longer found. The purpose of this study is to review the species that have been found in Indonesia, and find the factors that affect conservation efforts to prevent the extinction of this plant. The results of the study succeeded in recording 221 species that had been found in Indonesia with 4 of them not having sufficient data so that named Rhododendron sp1, R. sp2, R. sp3 and R. sp4 by local residents. Four species were confirmed to be extinct, and most of the ex-situ conservation efforts unsuccessful. The results of this study show that there is need for cooperation between the government and residents around the Rhododendron growing location in an effort to preserve this plant. Researchers are also expected to pay more attention to this plant considering it has bioactive compounds with very high pharmacological properties.
Co-Authors Aminuyati Antonia Fransiska Laka Damiana Nofita Birhi Irma Nur Sukmawati Sumarno Retro Prawiro