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All Journal JBIO: jurnal biosains (the journal of biosciences) Jurnal Biologi dan Pembelajarannya (JBP)
Sulistyarini Gultom, Endang
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology

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Uji Aktivitas Antibakteri Dari Ekstrak Daun Sirih Terhadap Bakteri Bacillus cereus Hasibuan, Ali Ahmatul Umri; Br Tarigan, Grace Amelia; Ulfah Rambe, Khadra; Tarigan, Sisilia Anabina; Sulistyarini Gultom, Endang
Jurnal Biologi dan Pembelajarannya (JB&P) Vol 11 No 1 (2024): APRIL 2024
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jbp.v11i1.21662

Abstract

This study elucidates the role of secondary metabolites in green betel leaves (Piper betle) as antibacterial agents against Bacillus cereus, a bacterium causing various foodborne illnesses in humans. The experimental research was conducted at the Biology Laboratory, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, utilizing Bacillus cereus bacterial isolates and extracting betel leaves using water. The measurement of inhibition zones was performed to evaluate the antibacterial activity of the betel leaf extract. The research results indicate the presence of an inhibition zone against Bacillus cereus growth, albeit categorized as weak. The measurements varied across repetitions, namely 2.6 mm, 3.35 mm, and 1.05 mm. The average of these three results was 3 mm.
Identification of Secondary Metabolites of Kirinyuh Leaves (Chromolaena odorata L.) as Antibacterial Using GS-MS grasella, martha; Sulistyarini Gultom, Endang
JURNAL BIOSAINS Vol. 11 No. 1 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i1.64589

Abstract

This study aims to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against pathogenic bacteria and to determine secondary metabolites in kirinyuh leaves (Chromolaena odorata) using the GC-MS (Gas Chromatography-Mass Spectroscopy) method. The test bacteria used were Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa. The extraction method was carried out by maceration using 96% ethanol solvent. The crude ethanol extract of kirinyuh leaves in this study used concentrations of 30%, 40%, 50%, 60% and 70%. Using a UV-VIS spectrophotometer, the most effective concentration in inhibiting and killing the growth of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa bacteria was a concentration of 70%, with an average OD (absorbance) value of -0.2029, -0.055, and -0.0523. Then secondary metabolite identification was carried out using GC-MS (Gas Chromatography-Mass Spectroscopy) ethanol-water (9:1). The test results showed that a concentration of 70% was the most effective in inhibiting and killing Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa bacteria. The content of secondary metabolites in the ethanol extract of kirinyuh leaves contains 33 secondary metabolite compounds, 6 of which are dominant compounds as antibacterials.
Co-Authors Br Tarigan, Grace Amelia grasella, martha Hasibuan, Ali Ahmatul Umri Tarigan, Sisilia Anabina Ulfah Rambe, Khadra