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Plasmonic wave assessment via optomechatronics system for biosensor application Abdullah, Muhammad Rosli; Harun, Noor Hasmiza; Ibrahim, Siti Noorjannah; Abdul Wahab, Azimah; Jamilan, Mohd Azerulazree
International Journal of Electrical and Computer Engineering (IJECE) Vol 14, No 2: April 2024
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijece.v14i2.pp1382-1389

Abstract

Transduction biosensor (mass-based, optical and electrochemical) involves analysis, recognition and amplification in the acquired sample. In this work, the plasmonic-based biosensor was employed without using tags. It is crucial to determine angles of Brewster (Ɵb) and critical (Ɵc) for generating plasmonic resonance (Ɵr). The objective is to verify a cost-effective plasmonic biosensor through Fresnel simulation and experimentation of a developed optomechatronics system. The borosilicate glass, Au and Air layers were simulated with the Winspall 3.02 simulator. The optomechatronics system consists of: 1-optics (650 nm laser, slit, polarizer, photodiode), 2-mechanical (bipolar stepper motors, gears, stages) and 3-electronics (PIC18F4550, liquid crystal display (LCD) and drivers). Later, the software performs angular interrogation by reading the reflected beam from a rotating prism at 0.1125. Experimentation to simulation accuracy indicates that percentage differences for Ɵr and Ɵc are 1% and 0.2%, respectively. In conclusion, excellence verification was successfully achieved between experimentation and simulation. It proved that the low-cost optomechatronics system is capable and reliable to be deployed for the biosensor application.
The optimization of self-assembled monolayer of thiols on screen-printed gold electrode Rahim, Nur Aina Syuhada; Harun, Noor Hasmiza; Abdullah, Muhammad Rosli; Jamilan, Mohd Azerulazree; Kamarudin, Balqis; Abdul Wahab, Azimah; Ibrahim, Siti Noorjannah; Jimat, Dzun Noraini
International Journal of Advances in Applied Sciences Vol 12, No 4: December 2023
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijaas.v12.i4.pp405-412

Abstract

The activated gold-modified electrode surface for self-assembled monolayer (SAM) is suitable for N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC), 16-mercaptohexadecanoic acid (16-MDA), and N-hydroxysuccinimide (NHS). The various substrates could potentially be used for surface functionalization that binds to one another. Using modified screen-printed gold electrodes, 16-MDA, EDC, and NHS, we developed a self-assembling monolayer. Different 16-MDA concentrations were applied to the surface of a screen-printed electrode surface to enhance the sensitivity of the working electrode. The impact of different EDC, NHS, and 16-MDA concentrations (0.4 M, 0.8 M, 1 M, 1.2 M, 1.4 M, and 1.8 M) and incubation times between 5 and 30 minutes were examined and compared. The binding surface of the screen-printed gold electrode was characterized using the differential pulse voltammetry (DPV) technique. It has been demonstrated that the substrate concentrations at 5 minutes and 30 minutes of incubation time used to have the highest surface coverage and electron transfer rates. This concentration would be utilized in the subsequent experiment to evaluate the binding of various bacterial species. These findings suggest that the SAM in a modified screen-printed gold electrode may be functionalized to detect microorganisms.