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All Journal Indonesian Journal of Life Sciences
Ratnasari, Nanda Risqia Pradana
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Medicine & Pharmacology

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Prediction Methods of the Protein Subcellular Localization: A Systematic Reviews Parikesit, Arli Aditya; Patricia, Gabriella; Ratnasari, Nanda Risqia Pradana
Indonesian Journal of Life Sciences 2019: IJLS Vol 01 No .02
Publisher : Indonesia International Institute for Life Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (392.033 KB) | DOI: 10.54250/ijls.v1i2.20

Abstract

The prediction of protein subcellular localization (SCL) has been a long-running challenge in bioinformatics. Protein SCL is crucial for a protein to exercise its functions properly. The reliance of protein localization on signaling peptides and the information available in gene ontology (GO) databases makes it possible to use computational approaches to predict protein SCL. SCL methods can be classified as either sequence-based or annotation-based. Machine learning algorithms and classifiers are used in protein SCL prediction tools. This review presents a list of protein SCL predictors published in the last 5 years.
Correlation Between miR-223 Expression and Rheumatoid Arthritis A, Angelika; Djohan, Carren Kwoknata; Julianto, Nadya Marcelina; Putri, Tiffanny Adora; Julietta, Vivi; Ratnasari, Nanda Risqia Pradana
Indonesian Journal of Life Sciences 2021: IJLS Vol 03 No .01
Publisher : Indonesia International Institute for Life Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (424.623 KB) | DOI: 10.54250/ijls.v3i1.120

Abstract

Patients diagnosed with rheumatoid arthritis (RA) show symptoms of joint affiliations. Focusing on the epigenetic factors leading to RA, the expression of several miRNAs are assumed to be one of the components influencing the cause of RA. Consequently, the increased expression of miR-223 is hypothesized to be involved in the pathogenesis of RA. This study aims to analyze the increased expression of miR-223 of RA patients in comparison with the normal and healthy representatives. Five methods from five different studies were involved in this review. The RNA was first isolated from heparinized venous blood or peripheral blood of RA patients and healthy controls. Reverse transcription was done to convert isolated RNA into cDNA and the expression of miR-223 was then measured using real-time PCR. The expression of miR-223 had exhibited a considerable increase in its concentration on RA patients when compared to healthy controls. Besides, the heightened concentration is taken upon consideration as miR-223 has a role in the regulation of the immune system and inflammatory responses.
Co-Authors A, Angelika Arli Aditya Parikesit Djohan, Carren Kwoknata Julianto, Nadya Marcelina Julietta, Vivi Patricia, Gabriella Putri, Tiffanny Adora