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All Journal Narra J Brawijaya Journal of Urology
Prasetyawan, Widiyanto
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Effects of metformin and silodosin as supplementary treatments to abiraterone on human telomerase reverse transcriptase (hTERT) level in metastatic castration-resistant prostate cancer (mCRPC) cells: An in vitro study Hidayatulla, Furqan; Andhika, Dimas P.; Prasetyawan, Widiyanto; Rahman, Zakaria A.; Pratama, Putu KD.; Hakim, Lukman
Narra J Vol. 4 No. 1 (2024): April 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i1.680

Abstract

The antiproliferative properties of metformin and silodosin have been observed in prostate cancer. Furthermore, it is hypothesized that the molecular pathways related to these drugs may impact the levels of human telomerase reverse transcriptase (hTERT) in prostate cancer cells. The aim of this study was to assess the effect of metformin and silodosin on the levels of hTERT in metastatic castration-resistant prostate cancer (mCRPC) cells. The present study employed an experimental design with a post-test-only control group.  This study utilized the PC3 cell line as a model for mCRPC. A viability experiment was conducted using the CCK-8 method to determine the inhibitory concentration (IC50) values of metformin, silodosin, and abiraterone acetate (AA) after a 72-hour incubation period of PC3 cells. In order to investigate the levels of hTERT, PC3 cells were divided into two control groups: a negative control and a standard therapy with AA. Additionally, three experimental combination groups were added: metformin with AA; silodosin with AA; and metformin, silodosin and AA. The level of hTERT was measured using sandwich ELISA technique. The difference in hTERT levels was assessed using ANOVA followed by a post hoc test. The IC50 values for metformin, silodosin, and AA were 17.7 mM, 44.162 mM, and 66.9 μM, respectively. Our data indicated that the combination of metformin with AA and the combination of metformin, silodosin and AA decreased the hTERT levels when compared to control, AA, and silodosin with AA. The administration of metformin resulted in a reduction of hTERT levels in the PC3 cell line, but the impact of silodosin on hTERT levels was not statistically significant compared to AA group.
Urachal Adenocarcinoma: A Case Report of Rare Tumor in Women Setya, Rindha Ilyasa; Prasetyawan, Widiyanto
Brawijaya Journal of Urology Vol. 6 No. 03 (2026): Brawijaya Journal of Urology
Publisher : Department of Urology, Faculty of Medicine, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/bjurology.2026.006.03.1

Abstract

Introduction. Urachal adenocarcinoma is an uncommon malignancy that typically presents in older men, and its recognition is often delayed because early symptoms are vague. This report is distinctive because it involves a young female patient with a mucinous colon-type immunophenotype, a presentation that is unusually atypical for urachal tumors and can easily be mistaken for a gastrointestinal primary. This rare combination of demographic and pathological features makes the case clinically significant, as it highlights specific diagnostic pitfalls that are not commonly emphasized in the literature. Case. A 40-year-old woman experienced recurrent dysuria and visible hematuria for one year. CT imaging revealed a mass at the bladder dome extending toward the umbilicus, raising suspicion for a urachal lesion. Following cystoscopic biopsy suggesting mucinous adenocarcinoma, the patient underwent surgical extirpation with partial cystectomy. Histopathology accompanied by immunohistochemistry demonstrated CK7 negativity with CK20, CDX2, and β-catenin positivity, an immunoprofile characteristic of colon-type urachal adenocarcinoma. Conclusion. Early cross-sectional imaging for persistent hematuria, close attention to masses located at the midline bladder dome, and the routine use of immunohistochemical markers to differentiate urachal from colorectal adenocarcinoma are essential steps to enhance diagnostic accuracy. Incorporating these strategies may prevent missed or delayed diagnoses in similarly atypical presentations of urachal adenocarcinoma.
Co-Authors Andhika, Dimas P. Hidayatulla, Furqan Lukman Hakim Pratama, Putu KD. Rahman, Zakaria A. Setya, Rindha Ilyasa