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All Journal 3BIO: Journal of Biological Science, Technology and Management Current Research on Biosciences and Biotechnology Makara Journal of Science
Isty Adhitya Purwasena
School Of Life Science And Technology, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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Indigenous Microbial Biostimulation for Microbial Enhanced Oil Recovery through Oil Degradation with Variation in Nutrent Concentrations Handaruni, Rika; Astuti, Dea Indriani; Purwasena, Isty Adhitya; Afifah, Lulu Nur
Makara Journal of Science Vol. 24, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Oil production in Indonesia has been declining since 2002, and this decline is incompatible with the high oil consumption in Indonesia. As such oil production in Indonesia should be improved. Biostimulation in microbial enhanced oil recovery involves environmental modification to stimulate microbial growth within a reservoir by adding limiting nutrients. Variation in C, N, and P concentrations injected to reservoirs can induce different responses from indigenous microbes and increase oil recovery. In this study, molasses, urea and diammonium phosphate were used as nutrients injected to a reservoir for biostimulation. Biological, physical and chemical characteristics after biostimulation were observed and bacterial growth was monitored up to 42 days. The physical characteristics observed were pH, oil viscosity, and interfacial tension. Gas chromatography mass spectrometry was performed to determine the chemical characteristics of oil. Results showed that the addition of nutrients at various concentrations yielded different production patterns of metabolites. The addition of urea and diammonium phosphate induced biosurfactants overproduction and increase hydrocarbon degradation of by bacteria. Therefore, hydrocarbons were degraded for the first 14 days, and polymerized again on days 14 to 42.
The effect of paraquat dichloride and carbosulfan on soil conditions and population dynamic of soil microbes in a cornfield: a case study in Sumedang, West Java Taufikurahman, Taufik; Aryantha, I Nyoman P; Purwasena, Isty Adhitya; Zahra, Muhandinni; Stefania, Marchia; Fitriyani, Azizah Nur Fitriyani
Current Research on Biosciences and Biotechnology Vol. 5 No. 2 (2024)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2024.5.2/BQCGURSN

Abstract

Paraquat dichloride (PC) is one of the herbicides active ingredients that is widely used by farmers to control various types of weeds, while carbosulfan (CS) is one of the insecticide’s active ingredients that is widely used to control various types of insect pests. The objective of this study is to analyze the impact of pesticides for PC and CS on the chemical and physical conditions of the soil and the dynamics of fungi and soil bacteria population in a corn field in Sumedang district, West Java. The experiment took place between August and October, during the dry season, concluding just as the rainy season began. The PC concentrations used were 0 mL/L, 3.33 mL/L, 4 mL/L, 4.66 mL/L and 5.33 mL/L, while the CS concentrations used were 0 mL/L, 0.5 mL/L, 1 mL/L, 1.5 mL/L and 2 mL/L. Soil sampling was conducted before and after the application of pesticides. The total abundance of fungi and bacteria was analyzed using the total plate count (TPC) method. The application of PC to the cornfield slightly decreased soil bulk density from 1.354 g/cm3 to 0.816 g/cm3, while the application of CS slightly increased soil bulk density. A decrease in bulk density is considered positive for the physical conditions of the soil for plant cultivation. In general, the result indicated that the application of PC and CS did not show a significant effect on the chemical characteristics of the soil, and also it did not affect the population of fungi and bacteria. Weather conditions, i.e. dry season (June-September) and rainy season (started from October) seem to affect more on soil conditions as well soil microbial population than the application of the pesticides.
Optimizing the Wet Fermentation of Ateng Coffee (Arabica) with the Addition of Yeast R1-TKSU and LAB (Leuconostoc suionicum) Inoculum Sabatina, Grace; Astuti, Dea Indriani; Purwasena, Isty Adhitya
3BIO: Journal of Biological Science, Technology and Management Vol. 6 No. 1 (2024)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2024.6.1.5

Abstract

Ateng coffee is an Arabica coffee that generally has a high selling value because of its better taste compared to other coffees. However, ateng coffee has poor post-harvest processing, resulting in low coffee quality and an impact on low coffee selling prices. In this study, optimization ofwet fermentation using yeast R1-TKSU and LAB (Leuconostoc suionicum) inoculums was used to improve the quality of ateng coffee. Treatment variations were fermentation with the addition of yeast, LAB, yeast : LAB 1:1, and there is also a control without the addition of inoculum. Sensory assessment showed that the best cupping score was the sample added by LAB with a total score of 86.5. The contents of malic acid, citric acid, lactic acid, sucrose, fructose, and glucose and ethanol which were analyzed by HPLC were found to be relatively stable ingreen coffee beans. Fermentation added inoculum affects the concentration of acetic acid. Amino acid analysed showed that overall the highest concentration of amino acids in green coffee beans was in the yeast : LAB 1:1 treatment. It can be concluded that controlled wet fermentation with the addition of yeast R1-TKSU and LAB (Leuconsotoc suionicum) inoculums can improve the quality of ateng coffee.  
Nanoemulsions of Biosurfactant and Cinnamon Essential Oil: A Promising Antimicrobial Approach for Oral Pathogens Isty Adhitya Purwasena; Anriansyah Renggaman; Dzulianur Mutsla; Nurul Wahyuni; Annisa Afiva; Dea Ajeng Pratiwi
3BIO: Journal of Biological Science, Technology and Management Vol. 8 No. 1 (2026): Vol 8 No 1 (2026)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2026.8.1.2

Abstract

Dental and oral care products often contain Sodium Lauryl Sulfate (SLS), which can cause allergic reactions and mucosal infection. Biosurfactants (BS) and cinnamon essential oil (CEO) are safer alternatives due to their antimicrobial properties. This study screens BS form five bacterial isolates, CEO, and their nanoemulsions prepared by low-energy method (NEL) and high- energy method (NEH) for inhibiting oral pathogens. Results showed that BS from Bacillus altitudinis were most effective against Candida albicans, while BS from Bacillus siamensis were most effective against Streptococcus mutans and Enterococcus faecalis. The minimum concentrations of CEO inhibiting C. albicans were 1900 ppm and both S. mutans and E. faecalis were 490 ppm. The minimum NEL and NEH concentration inhibiting C. albicans was 3.125 ppm BS+ 175 ppm CEO and 6.25 ppm BS + 350 ppm CEO, respectively. The minimum NEL concentration inhibiting S. mutans and E. faecalis was 7.82 ppm BS + 120 ppm CEO and 1.95 ppm BS + 120 ppm CEO, while the minimum concentration of both NEH was 3.125 ppm BS+ 175 ppm CEO. These findings suggest that BS combined with CEO in nanoemulsions are effective in inhibiting oral pathogens and can serve as promising alternatives to SLS.  
Co-Authors Afifah, Lulu Nur Annisa Afiva Anriansyah Renggaman Dea Ajeng Pratiwi Dea Indriani Astuti Dzulianur Mutsla Fitriyani, Azizah Nur Fitriyani Handaruni, Rika I Nyoman P Aryantha Nurul Wahyuni Sabatina, Grace Stefania, Marchia Taufikurahman, Taufik Zahra, Muhandinni