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Unravelling The Diversity of Cherry Tomato (Solanum Lycopersicum Var. Cerasiforme) Seed Microbes and Their Effect on Seed Health Aumentado, Herbert Dustin; Bengoa, Jennelyn; Balendres, Mark Angelo
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.84919

Abstract

Healthy seeds are the foundation of healthy plants. Planting healthy seeds contributes to securing crop productivity and seed germplasm conservation. In this study, we have identified microbes associated with seeds of three cherry tomato genotypes and demonstrated their negative effect on general seed health. Through a combined morpho-cultural and molecular characterisation (using multi-loci analysis of the ITS, β-tubulin, tef1α, and gapdh gene regions for fungi and 16s rDNA for bacteria), we have identified three fungi (Nigrospora sphaerica, N. lacticolonia, and Curvularia aeria), and two bacteria (Citrobacter freundii, and Stenotrophomonas maltophilia) from healthy-looking tomato seeds. These fungi and bacteria, through seed-soaked-inoculation, caused seed discoloration, lesions, and low germination. To our knowledge, these are the first reports of Nigrospora sphaerica, N. lacticolonia, Curvularia aeria, Citrobacter freundii, and Stenotrophomonas maltophilia on tomato seeds and demonstrated their negative impact on seed health. Seed treatment and interventions are needed to negate the possible effect of these microbes. Future studies on possible seed transmission are warranted. 
Medicines in medicines: Challenges and Prospects in Drug Discovery from Fungal Endophytes of Medicinal Plants in Southeast Asia: Challenges and Prospects in Drug Discovery from Fungal Endophytes of Medicinal Plants Guerrero, Jonathan Jaime G.; General, Mheljor; Balendres, Mark Angelo; Tan, Mario; Buiza, Marielle; Huyop, Fahrul
Journal of Tropical Life Science Vol. 15 No. 2 (2025): In Press
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/xvfdnn97

Abstract

Fungal endophytes engage in a balanced symbiotic continuum with their host plants, from mutualism to commensalism and parasitism. These host-endophyte interactions play a pivotal role in shaping the biosynthetic and chemical space of the host plants and their associated mycobiomes, resulting in a wealth of biologically active natural products that are attractive for drug discovery. Endophytic fungi in medicinal plants continue to attract attention, highlighting a holistic view of plant-based therapeutics that considers the plant and its endophytic partners as sources of medicinal compounds. In this review, we assembled recent Southeast Asian studies related to fungal endophytes from plants with traditional medicinal uses and their potential biomedical applications. Here, we present a comprehensive analysis of the interactions within the region, identifying prolific fungal classes that produce compounds of biomedical significance. This analysis is based on a consolidated dataset of over 1,300 interactions. We noted an association between plant-endophyte-synthesized compounds and their related chemistry. However, the majority are preliminary and lack comprehensive follow-through research. While fungal endophytes in the region produce important compounds, their applicability in drug discovery necessitates extensive long-term studies.