This study aimed to investigate the relationship between leg length, waist flexibility, and arm muscle strength and Gyaku Tsuki's punch speed in karate martial arts. A total of 30 karateka (age = 16.4 ± SD = 0.626) participated in the study. Leg length was measured using anthropometric methods, waist flexibility was measured by sit and reach tests, arm muscle strength was measured by push-up tests, and Gyaku Tsuki's punch speed was measured using Kinovea software. The results showed that leg length, waist flexibility, and arm muscle strength had a significant positive relationship with Gyaku Tsuki's punch speed (p = 0.000 0.005). This research can be the basis for further research in the field of kinematics and movement kinetics in the context of martial arts.